Fluocell: A Software Package for Analysis and Visualization of Ratiometric Fluorescence Image of Live Cells
Fluocell is a software package for the analysis and visualization of fluorescence images of live cells, with applications in migration and cancer invasion. The main package contains a visualization and quantification tool for fluorescence intensity and FRET ratio images. Optional modules of diffusion analysis and polarity analysis can be obtained upon request. This package is mainly written in MATLAB. It is being developed by a group of researchers in the "Molecular Engineering for Single Cell Imaging and Reprogramming" at University of California, San Diego. As developers, we are open for collaboration on using the software or developing additional features. If you find this software package of interest or useful for your work, please take the time to cite our work and write an email to tell us, since positive feedbacks are important to secure continuous support for this software.
Citations for publication:
- Lu S et al. 2008 PLoS Computational Biology, The spatiotemporal pattern of Src activation at lipid rafts revealed by diffusion-corrected FRET imaging.
- Lu S et al. 2011 PLoS ONE, Computational analysis of the spatiotemporal coordination of polarized PI3K and Rac1 activities in micro-patterned live cells.
Tested with operation systems: Windows 10, 64 bit MacOS 10, 11.1, 64 bit Requires Java 8.0 - 15.0 or similar versions Requires MATLAB, tested in MATLAB 2013-2019
For starters, the Fluocell User's Guide is in the doc/ folder. The installation guide is under the section "Installation and Usage". After installation, please go through the whole "User's Guide" to get familiar with Fluocell (This takes about 4-8 hours). Then you may go ahead and try with your own data.
Email Contact Shaoying (Kathy) Lu : shaoying.lu@gmail.com