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Bismark is a program to map bisulfite treated sequencing reads to a genome of interest and perform methylation calls in a single step.

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singularity-bismark

Singularity recipe for bismark.

Installing the container on Bridges 2

Copy the

  • SIF file
  • and the bismark script

to /opt/packages/bismark/0.22.3

Copy the file modulefile.lua to /opt/modulefiles/bismark as 0.22.3.lua.

Building the image using the recipe

To build the image locally

Run the script build.sh to build image locally.

bash ./build.sh

To build the image remotely

Run the script rbuild.sh to build image remotely.

bash ./rbuild.sh

To run tests

To run the available tests, run the command

bash ./test.sh

Copyright © 2020-2021 Pittsburgh Supercomputing Center. All Rights Reserved.

The Biomedical Applications Group at the Pittsburgh Supercomputing Center in the Mellon College of Science at Carnegie Mellon University.

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Bismark is a program to map bisulfite treated sequencing reads to a genome of interest and perform methylation calls in a single step.

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