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ReannTE | ||
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Last Update: 2014 08 06 | ||
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Scripts to help transposable elements consensus sequences curation | ||
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ReannTE_FilterLow.pl | ||
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WHAT IT DOES: | ||
This script uses Repeat Masker to mask low complexity / simple repeats of the input fasta file | ||
(for example, RepeatScout output) | ||
It eliminates the ones that are more than XX% masked (-p option) | ||
2 fasta outputs: retained sequences and rejected sequences | ||
perl <scriptname.pl> -i <fa> [-r <RMpath>] [-p <XX> | ||
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MANDATORY ARGUMENTS: | ||
-i <fa> => fasta file | ||
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[OPTIONAL ARGUMENTS]: | ||
-r <path> => path = localisation of repeat masker software | ||
if no path provided, path = /home/software/RepeatMasker | ||
-p <XX> => XX = threshold, in % (default = 80%). It sets the minimum low complexity masked % required to eliminate the sequence | ||
REQUIREMENTS: | ||
- Repeat Masker software, crossmatch engine | ||
- Bioperl (Bio::DB::Fasta, Bio::SeqIO) | ||
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