RNA-seq pipeline
This pipeline includes quality assessment and filtering, alignment, and count table generation. Specifically,
- Quality assess sequences with FastQC
- Remove adapters and filter low quality sequences with AdapterRemoval
- Align to reference genome with STAR
- Quality filter alignments with samtools
view
- Quality assess alignments with samtools
flagstat
and/or PicardCollectRnaSeqMetrics
- Count reads in genes with Subread
featureCounts
See our tutorial for further details.