Protein Parameter Analysis with ProtParam

This repository contains a Python script for analyzing protein parameters using Biopython's ProtParam module. The script calculates various physicochemical properties of proteins from a FASTA file and writes the results to an output text file.

Requirements

Python 3.x

Biopython (install via pip install biopython)

Input

Place your protein sequences in a FASTA format file named protein.fasta inside a directory. Run the script: python3 protparam_analysis.py The script will analyze each protein sequence, calculate its parameters, and write the results to protparam_analysis.txt in the directory.

Output

protparam_analysis.txt: Contains the calculated parameters for each protein sequence, including count and percentage of amino acids, molecular weight, aromaticity, isoelectric point, secondary structure fraction, GRAVY value, instability index, and flexibility.

Citation

If you are using the protparam_analysis.py script for your research, please consider citing it as follows: Sharma, R. (2024).protparam_analysis.py. Retrieved from https://github.com/BioinfoRhythm/protparam_analysis.py

Acknowledgments

Biopython developers for providing the ProtParam module.