Updates Over Time
Author: @ET 4/5/22 🐐
Edited: @MB 4/21/23
- Added MaRS GitHub Folder Structure diagram to readme
The emergence of resistance to all currently available antimalarial drugs in multiple regions of the world represents a global public health challenge. In order to monitor and address this situation, faster and more effective surveillance tools are required to track and monitor the emergence and evolution of drug resistance in malaria. The Malaria Resistance Surveillance (MaRS) project aims to address this challenge by collating and mapping genetic polymorphisms associated with drug resistance in P. falciparum malaria around the world.
Data for this project is generated using a targeted amplicon deep sequencing (TADS) approach in an effort to collect single nucleotide polymorphisms (SNPs) on all currently known drug resistance associated malaria genes from travelers returning to the US from overseas, as well as samples actively collected in collaboration with partners from other countries.
This projects aims to:
- ✅ Share all our laboratory and analysis protocols publicly
- ✅ Transfer these methods where they are most needed (malaria endemic countries)
- ✅ Standardize the entire process, from sample collection to report generation
Please see the Illumina Targeted Amplicon Deep Sequencing (TADS) Lab Protocol for the most up to date standard operating procedures (SOPs).
Two analysis workflows are available and actively being developed:
This MaRS repo is organized into the structure shown in Figure 1.
Figure 1. MaRS Project GitHub Folder Structure
Data for this project can be found at the following link NCBI BioProject. Collaborators are encouraged to submit their own data using the same NCBI BioProject
If you would like to contribute to this project, please Contact Us.
If you end up using any of the MaRS project workflows, please cite this study:
Next-Generation Sequencing and Bioinformatics Protocol for Malaria Drug Resistance Marker Surveillance. Talundzic E, Ravishankar S, Kelley J, Patel D, Plucinski M, Schmedes S, Ljolje D, Clemons B, Madison-Antenucci S, Arguin PM, Lucchi NW, Vannberg F, Udhayakumar V. Antimicrob Agents Chemother. 2018 Mar 27;62(4). pii: e02474-17. doi: 10.1128/AAC.02474-17. Print 2018 April
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