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2 changes: 1 addition & 1 deletion docs/build/html/_sources/contents.rst.txt
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Expand Up @@ -11,7 +11,7 @@ To provide a fair baseline while maintaining easy usability, per default we run
The tools included here are the most widely used and well maintained among AS event detection tools. If you would like to include your tool in the pipeline please let us know.
We hope this collection can be a starting point for future benchmarking approaches and quality control.

.. figure:: /img/Screenshot_dicast.png
.. figure:: /img/pipeline.png


What is DICAST?
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22 changes: 11 additions & 11 deletions docs/build/html/_sources/further-information/example-files.rst.txt
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Expand Up @@ -35,15 +35,15 @@ Running multiple mapping tools (E.g., STAR, HISAT2 and bbmap)
.. prompt:: bash

cd /path/to/DICAST/input
wget http://ftp.ensembl.org/pub/release-104/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
wget http://ftp.ensembl.org/pub/release-105/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
gunzip Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz

6. Download or copy the genome gtf annotation into the `input` folder. Dont't forget to uncompress it. E.g.:

.. prompt:: bash

wget http://ftp.ensembl.org/pub/release-104/gtf/homo_sapiens/Homo_sapiens.GRCh38.104.gtf.gz
gunzip Homo_sapiens.GRCh38.104.gtf.gz
wget http://ftp.ensembl.org/pub/release-105/gtf/homo_sapiens/Homo_sapiens.GRCh38.105.gtf.gz
gunzip Homo_sapiens.GRCh38.105.gtf.gz

7. Download or copy the fastq files you want to align into the `/path/to/DICAST/input/controldir/fastqdir`. Note: we support only paired-end RNA-Seq - fastq files have to be in pairs.

Expand All @@ -60,7 +60,7 @@ In the `config.sh` file edit the following lines:

read_length=76
fastaname=Homo_sapiens.GRCh38.dna.primary_assembly.fa
gtfname=Homo_sapiens.GRCh38.104.gtf
gtfname=Homo_sapiens.GRCh38.105.gtf

9. List the mapping tools you want to run:

Expand Down Expand Up @@ -119,22 +119,22 @@ Running multiple alternative splicing event detection tools (E.g., MAJIQ and Whi
.. prompt:: bash

cd /path/to/DICAST/input
wget http://ftp.ensembl.org/pub/release-104/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
wget http://ftp.ensembl.org/pub/release-105/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
gunzip Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz

6. Download or copy the genome annotation file into the `input` folder. Dont't forget to uncompress it. E.g.:

.. prompt:: bash

wget http://ftp.ensembl.org/pub/release-104/gtf/homo_sapiens/Homo_sapiens.GRCh38.104.gtf.gz
gunzip Homo_sapiens.GRCh38.104.gtf.gz
wget http://ftp.ensembl.org/pub/release-105/gtf/homo_sapiens/Homo_sapiens.GRCh38.105.gtf.gz
gunzip Homo_sapiens.GRCh38.105.gtf.gz

7. Download or copy the genome gff3 annotation into the `input` folder (for MAJIQ). Dont't forget to uncompress it. E.g.:

.. prompt:: bash

wget http://ftp.ensembl.org/pub/release-104/gff3/homo_sapiens/Homo_sapiens.GRCh38.104.gff3.gz
gunzip Homo_sapiens.GRCh38.104.gff3.gz
wget http://ftp.ensembl.org/pub/release-105/gff3/homo_sapiens/Homo_sapiens.GRCh38.105.gff3.gz
gunzip Homo_sapiens.GRCh38.105.gff3.gz

8. Download or copy the fastq files you want to use into the `/path/to/DICAST/input/controldir/fastqdir`. Note: we support only paired-end RNA-Seq - fastq files have to be in pairs.

Expand All @@ -153,8 +153,8 @@ In the `config.sh` file edit the following lines:

read_length=76
fastaname=Homo_sapiens.GRCh38.dna.primary_assembly.fa
gtfname=Homo_sapiens.GRCh38.104.gtf
gffname=Homo_sapiens.GRCh38.104.gff3
gtfname=Homo_sapiens.GRCh38.105.gtf
gffname=Homo_sapiens.GRCh38.105.gff3

11. List the mapping tools you want to run:

Expand Down
4 changes: 2 additions & 2 deletions docs/build/html/_sources/get-started/config.rst.txt
Original file line number Diff line number Diff line change
Expand Up @@ -85,12 +85,12 @@ ncores
Input Parameters
^^^^^^^^^^^^^^^^
asimulator_gtf
| the genome gtf annotation that you use to simulate the data. Default: 'Homo_sapiens.GRCh38.104.gtf'.
| the genome gtf annotation that you use to simulate the data. Default: 'Homo_sapiens.GRCh38.105.gtf'.
fastaname
| the genome reference file. Default: 'Homo_sapiens.GRCh38.dna.primary_assembly.fa'.
gtfname
| the genome gtf annotation that you use for mapping and alternative splicing analysis. If you’re using ASimulatoR, leave this as ASimulatoR.gtf.
gffname
| the genome gff3 annotation that you use for mapping and alternative splicing analysis. If you’re using ASimulatoR, leave this as ASimulatoR.gff3.
The reference genome, annotation file and gff3 files could be downloaded from `Ensembl <http://ftp.ensembl.org/pub/release-104/>`_.
The reference genome, annotation file and gff3 files could be downloaded from `Ensembl <http://ftp.ensembl.org/pub/release-105/>`_.
Original file line number Diff line number Diff line change
Expand Up @@ -106,7 +106,7 @@ ASimulatoR Parameters

asimulator_gtf
| Name of the file in the input directory used by ASimulatoR to generate new transcripts
| Example: ``Homo_sapiens.GRCh38.104.gtf``
| Example: ``Homo_sapiens.GRCh38.105.gtf``

Input Parameters
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2 changes: 1 addition & 1 deletion docs/build/html/_sources/get-started/run.rst.txt
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Expand Up @@ -8,9 +8,9 @@ Run your analysis
.. toctree::
:maxdepth: 2

run/gui
run/dicast-cli
run/one_tool
run/gui
run/outputs


Expand Down
4 changes: 2 additions & 2 deletions docs/build/html/_sources/get-started/run/dicast-cli.rst.txt
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@@ -1,5 +1,5 @@
Run DICAST via Command Line Interface (depreciated)
===================================================
Run DICAST via Command Line Interface
======================================

In this section we will explain how to use DICAST to run a whole pipeline on the terminal alone.

Expand Down
4 changes: 2 additions & 2 deletions docs/build/html/_sources/get-started/run/gui.rst.txt
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@@ -1,5 +1,5 @@
Run DICAST with a graphical user interface
================================================
Run DICAST with a graphical user interface (depreciated)
========================================================

.. toctree::
:maxdepth: 1
Expand Down
2 changes: 1 addition & 1 deletion docs/build/html/_sources/get-started/run/one_tool.rst.txt
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Expand Up @@ -16,7 +16,7 @@ If you have already built the image with <tool>:<tag> (see the :doc:`docker setu
# If you are using our directory structure for your input and are in the dockers directory:
# Add the --rm flag to remove container, after run.

docker run -v ./:/MOUNT --user $(id -u):$(id -g) --rm gsnap:0.1
docker run -v $(pwd):/MOUNT --user $(id -u):$(id -g) --rm gsnap:0.1


Troubleshooting
Expand Down
2 changes: 1 addition & 1 deletion docs/build/html/_sources/get-started/run/outputs.rst.txt
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Expand Up @@ -38,7 +38,7 @@ DICAST also provides a :guilabel:`output/<astoolname>-output/<Fastq-filename>_ou

------------------------------

DICAST also outputs an upset plot for each ``Fastq-filename``-``mapping_tool`` combination.
DICAST also outputs an UpSet plot for each ``Fastq-filename``-``mapping_tool`` combination.

.. figure:: ../../img/upset_plot.png

Expand Down
22 changes: 10 additions & 12 deletions docs/build/html/_sources/get-started/setup/docker.rst.txt
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Expand Up @@ -64,9 +64,18 @@ We support Docker version 19 and above.

docker-compose --version

2. Pull docker images (Not needed with snakemake)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

Should the tool you intended to run, not build locally, it's also possible to pull them from DICAST's dockerhub repository at: https://hub.docker.com/repository/docker/dicastproj/dicast


2. Build docker images (Optional)
.. prompt:: bash $

docker pull dicastproj/dicast:tagname


3. Build docker images (For Developers)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
While the steps described in this section are handled by DICAST's graphical interface, it can also be accessed via command line, for more control.

Expand Down Expand Up @@ -101,17 +110,6 @@ Where <tool> needs to be replaced with one or more of the following tools:

bbmap, contextmap, crac, dart, gsnap, hisat, mapsplice, minimap, segemehl, star, subjunc, asgal, aspli, eventpointer, irfinder, majiq, sgseq, spladder, whippet


3. Pull docker images (Fail safe)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

Should the tool you intended to run, not build locally, it's also possible to pull them from DICAST's dockerhub repository at: https://hub.docker.com/repository/docker/dicastproj/dicast


.. prompt:: bash $

docker pull dicastproj/dicast:tagname

4. Other helpful commands
^^^^^^^^^^^^^^^^^^^^^^^^^
To gracefully stop a running docker container (If perhaps snakemake's process had to be killed):
Expand Down
8 changes: 4 additions & 4 deletions docs/build/html/contents.html
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Expand Up @@ -67,13 +67,13 @@
</ul>
</li>
<li class="toctree-l1"><a class="reference internal" href="get-started/run.html">Run your analysis</a><ul>
<li class="toctree-l2"><a class="reference internal" href="get-started/run/gui.html">Run DICAST with a graphical user interface</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="get-started/run/dicast-cli.html">Run DICAST via Command Line Interface</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="get-started/run/dicast-cli.html">Run DICAST via Command Line Interface (depreciated)</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="get-started/run/one_tool.html">Run one specific tool via Docker</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="get-started/run/one_tool.html">Run one specific tool via Docker</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="get-started/run/gui.html">Run DICAST with a graphical user interface (depreciated)</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="get-started/run/outputs.html">DICAST Outputs</a></li>
Expand Down Expand Up @@ -153,7 +153,7 @@ <h1>Welcome to DICAST!<a class="headerlink" href="#welcome-to-dicast" title="Per
<p>The tools included here are the most widely used and well maintained among AS event detection tools. If you would like to include your tool in the pipeline please let us know.
We hope this collection can be a starting point for future benchmarking approaches and quality control.</p>
<figure class="align-default">
<img alt="_images/Screenshot_dicast.png" src="_images/Screenshot_dicast.png" />
<img alt="_images/pipeline.png" src="_images/pipeline.png" />
</figure>
</section>
<section id="what-is-dicast">
Expand Down
6 changes: 3 additions & 3 deletions docs/build/html/further-information/about.html
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Expand Up @@ -67,13 +67,13 @@
</ul>
</li>
<li class="toctree-l1"><a class="reference internal" href="../get-started/run.html">Run your analysis</a><ul>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/gui.html">Run DICAST with a graphical user interface</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/dicast-cli.html">Run DICAST via Command Line Interface</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/dicast-cli.html">Run DICAST via Command Line Interface (depreciated)</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/one_tool.html">Run one specific tool via Docker</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/one_tool.html">Run one specific tool via Docker</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/gui.html">Run DICAST with a graphical user interface (depreciated)</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/outputs.html">DICAST Outputs</a></li>
Expand Down
28 changes: 14 additions & 14 deletions docs/build/html/further-information/example-files.html
Original file line number Diff line number Diff line change
Expand Up @@ -68,13 +68,13 @@
</ul>
</li>
<li class="toctree-l1"><a class="reference internal" href="../get-started/run.html">Run your analysis</a><ul>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/gui.html">Run DICAST with a graphical user interface</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/dicast-cli.html">Run DICAST via Command Line Interface</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/dicast-cli.html">Run DICAST via Command Line Interface (depreciated)</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/one_tool.html">Run one specific tool via Docker</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/one_tool.html">Run one specific tool via Docker</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/gui.html">Run DICAST with a graphical user interface (depreciated)</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/outputs.html">DICAST Outputs</a></li>
Expand Down Expand Up @@ -176,13 +176,13 @@ <h2>Running multiple mapping tools (E.g., STAR, HISAT2 and bbmap)<a class="heade
<li><p>Download or copy the genome fasta file into the <cite>input</cite> folder. Dont’t forget to uncompress it. E.g.:</p></li>
</ol>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1"><span class="nb">cd</span> /path/to/DICAST/input</span>
<span class="prompt1">wget http://ftp.ensembl.org/pub/release-104/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz</span>
<span class="prompt1">wget http://ftp.ensembl.org/pub/release-105/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz</span>
<span class="prompt1">gunzip Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz</span>
</pre></div></div><ol class="arabic simple" start="6">
<li><p>Download or copy the genome gtf annotation into the <cite>input</cite> folder. Dont’t forget to uncompress it. E.g.:</p></li>
</ol>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1">wget http://ftp.ensembl.org/pub/release-104/gtf/homo_sapiens/Homo_sapiens.GRCh38.104.gtf.gz</span>
<span class="prompt1">gunzip Homo_sapiens.GRCh38.104.gtf.gz</span>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1">wget http://ftp.ensembl.org/pub/release-105/gtf/homo_sapiens/Homo_sapiens.GRCh38.105.gtf.gz</span>
<span class="prompt1">gunzip Homo_sapiens.GRCh38.105.gtf.gz</span>
</pre></div></div><ol class="arabic simple" start="7">
<li><p>Download or copy the fastq files you want to align into the <cite>/path/to/DICAST/input/controldir/fastqdir</cite>. Note: we support only paired-end RNA-Seq - fastq files have to be in pairs.</p></li>
<li><p>Go to <cite>/path/to/DICAST/scripts</cite> and edit <cite>config.sh</cite> according to your run (see <a class="reference internal" href="../get-started/config/general_parameters.html"><span class="doc">How to change your config.sh file</span></a>):</p></li>
Expand All @@ -196,7 +196,7 @@ <h2>Running multiple mapping tools (E.g., STAR, HISAT2 and bbmap)<a class="heade
}
</style><span class="prompt2">read_length=76</span>
<span class="prompt2">fastaname=Homo_sapiens.GRCh38.dna.primary_assembly.fa</span>
<span class="prompt2">gtfname=Homo_sapiens.GRCh38.104.gtf</span>
<span class="prompt2">gtfname=Homo_sapiens.GRCh38.105.gtf</span>
</pre></div></div><ol class="arabic simple" start="9">
<li><p>List the mapping tools you want to run:</p></li>
</ol>
Expand Down Expand Up @@ -236,18 +236,18 @@ <h2>Running multiple alternative splicing event detection tools (E.g., MAJIQ and
<li><p>Download or copy the genome fasta file into the <cite>input</cite> folder. Dont’t forget to uncompress it. E.g.:</p></li>
</ol>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1"><span class="nb">cd</span> /path/to/DICAST/input</span>
<span class="prompt1">wget http://ftp.ensembl.org/pub/release-104/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz</span>
<span class="prompt1">wget http://ftp.ensembl.org/pub/release-105/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz</span>
<span class="prompt1">gunzip Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz</span>
</pre></div></div><ol class="arabic simple" start="6">
<li><p>Download or copy the genome annotation file into the <cite>input</cite> folder. Dont’t forget to uncompress it. E.g.:</p></li>
</ol>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1">wget http://ftp.ensembl.org/pub/release-104/gtf/homo_sapiens/Homo_sapiens.GRCh38.104.gtf.gz</span>
<span class="prompt1">gunzip Homo_sapiens.GRCh38.104.gtf.gz</span>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1">wget http://ftp.ensembl.org/pub/release-105/gtf/homo_sapiens/Homo_sapiens.GRCh38.105.gtf.gz</span>
<span class="prompt1">gunzip Homo_sapiens.GRCh38.105.gtf.gz</span>
</pre></div></div><ol class="arabic simple" start="7">
<li><p>Download or copy the genome gff3 annotation into the <cite>input</cite> folder (for MAJIQ). Dont’t forget to uncompress it. E.g.:</p></li>
</ol>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1">wget http://ftp.ensembl.org/pub/release-104/gff3/homo_sapiens/Homo_sapiens.GRCh38.104.gff3.gz</span>
<span class="prompt1">gunzip Homo_sapiens.GRCh38.104.gff3.gz</span>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1">wget http://ftp.ensembl.org/pub/release-105/gff3/homo_sapiens/Homo_sapiens.GRCh38.105.gff3.gz</span>
<span class="prompt1">gunzip Homo_sapiens.GRCh38.105.gff3.gz</span>
</pre></div></div><ol class="arabic simple" start="8">
<li><p>Download or copy the fastq files you want to use into the <cite>/path/to/DICAST/input/controldir/fastqdir</cite>. Note: we support only paired-end RNA-Seq - fastq files have to be in pairs.</p></li>
<li><p>Download or copy the bam files you want to use into the <cite>/path/to/DICAST/input/controldir/bamdir</cite>.</p></li>
Expand All @@ -258,8 +258,8 @@ <h2>Running multiple alternative splicing event detection tools (E.g., MAJIQ and
</pre></div></div><p>In the <cite>config.sh</cite> file edit the following lines:</p>
<div class="highlight-default notranslate"><div class="highlight"><pre><span class="prompt1"><span class="nv">read_length</span><span class="o">=</span><span class="m">76</span></span>
<span class="prompt1"><span class="nv">fastaname</span><span class="o">=</span>Homo_sapiens.GRCh38.dna.primary_assembly.fa</span>
<span class="prompt1"><span class="nv">gtfname</span><span class="o">=</span>Homo_sapiens.GRCh38.104.gtf</span>
<span class="prompt1"><span class="nv">gffname</span><span class="o">=</span>Homo_sapiens.GRCh38.104.gff3</span>
<span class="prompt1"><span class="nv">gtfname</span><span class="o">=</span>Homo_sapiens.GRCh38.105.gtf</span>
<span class="prompt1"><span class="nv">gffname</span><span class="o">=</span>Homo_sapiens.GRCh38.105.gff3</span>
</pre></div></div><ol class="arabic simple" start="11">
<li><p>List the mapping tools you want to run:</p></li>
</ol>
Expand Down
6 changes: 3 additions & 3 deletions docs/build/html/further-information/faq.html
Original file line number Diff line number Diff line change
Expand Up @@ -68,13 +68,13 @@
</ul>
</li>
<li class="toctree-l1"><a class="reference internal" href="../get-started/run.html">Run your analysis</a><ul>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/gui.html">Run DICAST with a graphical user interface</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/dicast-cli.html">Run DICAST via Command Line Interface</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/dicast-cli.html">Run DICAST via Command Line Interface (depreciated)</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/one_tool.html">Run one specific tool via Docker</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/one_tool.html">Run one specific tool via Docker</a><ul class="simple">
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/gui.html">Run DICAST with a graphical user interface (depreciated)</a><ul class="simple">
</ul>
</li>
<li class="toctree-l2"><a class="reference internal" href="../get-started/run/outputs.html">DICAST Outputs</a></li>
Expand Down

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