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Some convenience functions used in CMETs automated reporting pipeline for microbial amplicon sequencing

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CMET-UGent/CMETNGS

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CMETNGS_package

R package with some convenience functions for CMETs NGS pipeline

Installation

library(devtools)
install_github("CMET-UGent/CMETNGS")

Available functions

For more details on a function call ?functionname

Function name Description
highqualgraphR export publication-grade graphics from ggplot2 object
makebargraphrawggplot2 make stacked bar graphs from preformatted taxonomy
preformattax preformat mothur taxonomies
coldiss fast plots of dissimilarity matrices
cbindPad function to combine unequal-length data frames (or vectors) into a dataframe
gg_color_hue function to generate ggplot-like color pallettes
myround alternative plotting function
fasta2dataframe bioconductor-based readout of (mothur formatted) sequences into a dataframe
KimPalettes Function to select a discrete color palette as created by Kim De Paepe
MakeEDABargraphs create some exploratory stacked bargraphs based upon mothur output files and phyloseq, as created by Tim Lacoere
df2fasta bioconductor-based inverse of fasta2dataframe (useful in case you did some filtering via fasta2dataframe but need a fasta output for downstream)
MakeExcelReport R function that takes absolute (!) paths to a shared, taxonomy and optional otureps fasta and automatically creates an excel file from it.
myround more robust rounding function
construct_phyloseq create a phyloseq object from mothur output, according to CMET SOP (different from phyloseq::import_mothur in that it works with the final files from our current SOP, with support for metadata)
Analyze_Sangers wrapper function for sangeranalyzeR package to deal with LGC/Tim Lacoere formatted data for 16S Sangers

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Some convenience functions used in CMETs automated reporting pipeline for microbial amplicon sequencing

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