R Markdown Scripts for analysis of Human Fetal and Adult GPCs

Link to Paper: Repression of developmental transcription factor networks triggers aging-associated gene expression in human glial progenitor cells

Processing of Fetal and Adult GPC Bulk RNA-seq
Bulk Differential Expression between CD140+ and P-/A+ or CD140- Fetal hGPCs
scRNA-Seq Analysis of CD140+ or P-/A+ Fetal Brain
Differential Expression between Adult and Fetal hGPCs
Identification of differentially active transcriptional regulators between adult and fetal hGPCs
Adult vs Fetal Repressor Isoform Analysis
Comparison of ESC- and iPSC-derived hGPCs to primary and adult hGPCs
qPCR and ICC Analysis of Adult Repressor OE in ESC- or iSPC-derived hGPCs
Processing of In Vitro Aging Overexperssion scRNA-seq
scRNA-Seq Analysis of ZNF274 or E2F6 Overexpression in C27 hGPCs
Differential Expression Analysis of Adult vs Fetal hGPC miRNAs