An isothermal shift assay for proteome scale drug-target identification

This release contains code and data used in the publication Ball, K.A., Webb, K.J., Coleman, S.J., Cozzolino, K.A., Jacobsen, J.,Jones, K.R., Stowell, M.H.B., Old, W.M. "An isothermal shift assay for proteome scale drug-target identification" Communications Biology (2019/2020).

Existing approaches for drug target identification employ complex workflows with low throughput. The isothermal shift assay (iTSA) is a proteome-wide method for identification of drug targets within lysates or living cells. iTSA uses a simplified experimental design with improved statistical power to detect small molecule binding targets across the proteome.