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29222727.txt
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29222727.txt
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PMID- 29222727
OWN - NLM
STAT- PubMed-not-MEDLINE
LR - 20171213
IS - 1817-406X (Print)
IS - 1817-406X (Linking)
VI - 58
IP - 1
DP - 2017 Dec 8
TI - A method for facilitating the seed germination of a mycoheterotrophic orchid,
Gastrodia pubilabiata, using decomposed leaf litter harboring a basidiomycete
fungus, Mycena sp.
PG - 59
LID - 10.1186/s40529-017-0214-6 [doi]
AB - BACKGROUND: Mycoheterotrophic plants are one of the most difficult plant groups
to conserve because they are entirely dependent on symbiotic fungi. Establishment
of viable culture systems would greatly aid their conservation. We describe a
simple culture system for the mycoheterotrophic orchid, Gastrodia pubilabiata,
that does not require laboratory facilities. The orchid is symbiotic with
leaf-litter-decomposing fungi. RESULTS: Gastrodia pubilabiata seeds were
incubated in plastic boxes or glass bottles filled with leaf litter collected
from the natural habitat of the species. Seed germination was observed after 35
days and seedling development followed. Fungal isolates from seedlings were
identified as Mycenaceae (Basidiomycota), a leaf-litter-decomposing mycorrhizal
fungus of Gastrodia species. CONCLUSION: Our method can be used to conserve
endangered mycoheterotrophic plants associated with leaf litter-decomposing fungi
efficiently, and can also serve as a model system for physiological and molecular
studies of such plants.
FAU - Higaki, Kana
AU - Higaki K
AD - Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, 840-8502, Japan.
FAU - Rammitsu, Kento
AU - Rammitsu K
AD - Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, 840-8502, Japan.
FAU - Yamashita, Yumi
AU - Yamashita Y
AD - Tsukuba Botanical Garden, National Museum of Nature and Science, 4-1-1 Amakubo,
Tsukuba, Ibaraki, 305-0005, Japan.
FAU - Yukawa, Tomohisa
AU - Yukawa T
AD - Tsukuba Botanical Garden, National Museum of Nature and Science, 4-1-1 Amakubo,
Tsukuba, Ibaraki, 305-0005, Japan.
FAU - Ogura-Tsujita, Yuki
AU - Ogura-Tsujita Y
AUID- ORCID: http://orcid.org/0000-0003-4877-4702
AD - Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, 840-8502, Japan.
ytsujita@cc.saga-u.ac.jp.
LA - eng
GR - 15H04417/Japan Society for the Promotion of Science/United States
GR - 17K07536/Japan Society for the Promotion of Science/United States
GR - Integrated analysis of natural histroy collections for conservation of highly
endangered species/A National Museum of Nature and Science research grant/United
States
PT - Journal Article
DEP - 20171208
PL - England
TA - Bot Stud
JT - Botanical studies
JID - 101321928
PMC - PMC5722783
OTO - NOTNLM
OT - Conservation
OT - Gastrodia
OT - Leaf litter-decomposing fungi
OT - Mycoheterotrophic plants
EDAT- 2017/12/10 06:00
MHDA- 2017/12/10 06:01
CRDT- 2017/12/10 06:00
PHST- 2017/09/05 00:00 [received]
PHST- 2017/12/01 00:00 [accepted]
PHST- 2017/12/10 06:00 [entrez]
PHST- 2017/12/10 06:00 [pubmed]
PHST- 2017/12/10 06:01 [medline]
AID - 10.1186/s40529-017-0214-6 [doi]
AID - 10.1186/s40529-017-0214-6 [pii]
PST - epublish
SO - Bot Stud. 2017 Dec 8;58(1):59. doi: 10.1186/s40529-017-0214-6.