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ekiernan committed Mar 4, 2020
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## Methods
# HCA Release March 2020 Methods
## Overview
This document details the Human Cell Atlas (HCA) Data Coordination Platform’s (DCP) methods for cell clustering, differential expression analyses, and data visualization used in the HCA March 2020 Data Release. Overall, individual DCP project loom files (available for download from the [DCP Data Portal]() were analyzed using Cumulus (v0.13.0), a single-cell analysis workflow ( [Li et al. 2019](https://www.biorxiv.org/content/10.1101/823682v1).
This document details the Human Cell Atlas (HCA) Data Coordination Platform’s (DCP) methods for cell clustering, differential expression analyses, and data visualization used in the HCA March 2020 Data Release. Overall, individual DCP project loom files (available for download from the [DCP Data Portal]()) were analyzed using Cumulus (v0.13.0), a single-cell analysis workflow ( [Li et al. 2019](https://www.biorxiv.org/content/10.1101/823682v1)).
Further details about the HCA March 2020 Data Release can be found on the [DCP Portal’s Data Release Page](INSERTLINK). A step-by-step guide for the entire release methods, including using the Cumulus workflow, is provided at [](INSERT LINK). Additionally, you can find techniques for manipulating and interacting with release files in [this tutorial](INSERT LINK).

## Project stratification and loom file preparation
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You can get hands-on experience with these methods by following our step-by-step guide (INSERT LINK). You can also find more details about each individual dataset by visiting INSERT RELEASE PAGE.


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