How to set the signal-to-noise ratio of simulated data?

[RecardoLyu]

Hi,
I'm learning how to use this software to generate a saries SMLM data that have different SNR. But I couldn't work it out. I'm not sure whether this function is implemented or not? I would be grateful if you'd let me know.

[DominiqueBourgeois]

Dear Recardo, There are several ways you can change the SNR in SMIS simulated data sets: => you can change the fluorescence background level => you can change laser intensities => you can change frametime => you can change pixel size => you can change numerical aperture of the objective => you can change EMCCD setup (EMCCD gain). => you can change the fluorescence quantum yield of your fluorophore (although this is not representative of real life) Basically you have to imagine that you are working with a real microscope. All the best, Dominique De: "RecardoLyu" ***@***.***> À: "DominiqueBourgeois/SMIS" ***@***.***> Cc: "Subscribed" ***@***.***> Envoyé: Jeudi 18 Janvier 2024 07:42:39 Objet: [DominiqueBourgeois/SMIS] How to set the signal-to-noise ratio of simulated data? (Issue #2) Hi, I'm learning how to use this software to generate a saries SMLM data that have different SNR. But I couldn't work it out. I'm not sure whether this function is implemented or not? I would be grateful if you'd let me know. — Reply to this email directly, [ #2 | view it on GitHub ] , or [ https://github.com/notifications/unsubscribe-auth/AWMTC6UV74YJBO76SZAKNGTYPC747AVCNFSM6AAAAABB7XTQKKVHI2DSMVQWIX3LMV43ASLTON2WKOZSGA4DONRSGM2TAOI | unsubscribe ] . You are receiving this because you are subscribed to this thread. Message ID: ***@***.***>