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cromwell error: java out of bounds #163

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shinlin77 opened this issue Apr 2, 2022 · 1 comment
Closed

cromwell error: java out of bounds #163

shinlin77 opened this issue Apr 2, 2022 · 1 comment

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@shinlin77
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I'm trying to run some of my own data. I keep running into this problem. Do you have any suggestions as to the problem?

********** NOTE: Picard's command line syntax is changing.


********** For more information, please see:
********** https://github.com/broadinstitute/picard/wiki/Command-Line-Syntax-Transition-For-Users-(Pre-Transition)


********** The command line looks like this in the new syntax:


********** CollectGcBiasMetrics -R /oak/stanford/scg/lab_mps/sl/chrombpnet/atac/8a8b155f-837c-4eb6-bd8e-6e3a590132db/call-gc_bias/shard-5/attempt-2/inputs/290321745/GRCh38_no_alt_analysis_set_GCA_000001405.15.fasta.gz -I ./Ventricular_Cardiomyocyte.nodup.no_chrM_MT.no_rg.bam -O Ventricular_Cardiomyocyte.nodup.no_chrM_MT.gc.txt -USE_JDK_DEFLATER TRUE -USE_JDK_INFLATER TRUE -VERBOSITY ERROR -QUIET TRUE -ASSUME_SORTED FALSE -CHART Ventricular_Cardiomyocyte.nodup.no_chrM_MT.gcPlot.pdf -S Ventricular_Cardiomyocyte.nodup.no_chrM_MT.gcSummary.txt


Exception in thread "main" java.lang.ArrayIndexOutOfBoundsException: Index 133338787 out of bounds for length 133275310
at picard.analysis.GcBiasMetricsCollector.addRead(GcBiasMetricsCollector.java:384)
at picard.analysis.GcBiasMetricsCollector.access$600(GcBiasMetricsCollector.java:48)
at picard.analysis.GcBiasMetricsCollector$PerUnitGcBiasMetricsCollector.addReadToGcData(GcBiasMetricsCollector.java:221)
at picard.analysis.GcBiasMetricsCollector$PerUnitGcBiasMetricsCollector.acceptRecord(GcBiasMetricsCollector.java:155)
at picard.analysis.GcBiasMetricsCollector$PerUnitGcBiasMetricsCollector.acceptRecord(GcBiasMetricsCollector.java:100)
at picard.metrics.MultiLevelCollector$AllReadsDistributor.acceptRecord(MultiLevelCollector.java:192)
at picard.metrics.MultiLevelCollector.acceptRecord(MultiLevelCollector.java:315)
at picard.analysis.CollectGcBiasMetrics.acceptRead(CollectGcBiasMetrics.java:172)
at picard.analysis.SinglePassSamProgram.makeItSo(SinglePassSamProgram.java:145)
at picard.analysis.SinglePassSamProgram.doWork(SinglePassSamProgram.java:84)
at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:305)
at picard.cmdline.PicardCommandLine.instanceMain(PicardCommandLine.java:103)
at picard.cmdline.PicardCommandLine.main(PicardCommandLine.java:113)
Traceback (most recent call last):
File "/home/sl/.conda/envs/encode-atac-seq-pipeline/bin/encode_task_gc_bias.py", line 143, in

@shinlin77
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I think this is due to incompatible genomes (bam versus what caper is using)

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