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Merge pull request #2 from Edinburgh-Genome-Foundry/dev
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Biopython v1.78
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veghp committed Sep 10, 2020
2 parents 2017bf8 + 49a615e commit b4af31d
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2 changes: 1 addition & 1 deletion .gitignore
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.pydevproject

# Temp files

.vscode/
*~

# Pipy codes
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14 changes: 5 additions & 9 deletions LICENCE.txt
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The MIT License (MIT)
[OSI Approved License]

The MIT License (MIT)
MIT License

Copyright (c) 2017 Edinburgh Genome Foundry

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copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in
all copies or substantial portions of the Software.
The above copyright notice and this permission notice shall be included in all
copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
THE SOFTWARE.
OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
26 changes: 15 additions & 11 deletions README.rst
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:target: https://travis-ci.org/Edinburgh-Genome-Foundry/GoldenHinges
:alt: Travis CI build status
.. image:: https://coveralls.io/repos/github/Edinburgh-Genome-Foundry/GoldenHinges/badge.svg?branch=master
:target: https://coveralls.io/github/Edinburgh-Genome-Foundry/GoldenHinges?branch=master
Golden Hinges (full documentation `here <https://edinburgh-genome-foundry.github.io/GoldenHinges/>`_) is a Python library to find sets
of overhangs (also called junctions, or protusions) for multipart DNA assembly
such as Golden Gate assembly.
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decomposition, in exterme cases where the original sequence does not allow
for such decomposition.
You can see Golden Hinges in action in this
`web demo <http://cuba.genomefoundry.org/design-overhangs>`_:
You can see Golden Hinges in action in this web demo:
`Design Golden Gate Overhangs <http://cuba.genomefoundry.org/design-overhangs>`_
Examples of use
----------------
---------------
Finding maximal overhangs sets
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Finding maximal overhang sets
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Let us compute a collection of overhangs, as large as possible, where
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Using experimental annealing data from Potapov 2018
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
`This study by Potapov *et al.* <https://www.biorxiv.org/content/early/2018/05/15/322297>`_
provides insightful data on overhangs annealing, in particular which overhangs
`This study by Potapov et al. <https://www.biorxiv.org/content/early/2018/05/15/322297>`_
provides insightful data on overhang annealing, in particular which overhangs
have weak general annealing power, and which pairs of overhangs have significant
"cross-talk". You can use the data in this paper via the Python
`tatapov <https://github.com/Edinburgh-Genome-Foundry/tatapov>`_ library
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the original solution will always be prefered, so no base change will be
suggested unless strictly necessary.
If the input record has `DnaChisel <https://github.com/Edinburgh-Genome-Foundry/DnaChisel>`_
If the input record has `DNA Chisel <https://github.com/Edinburgh-Genome-Foundry/DnaChisel>`_
annotations such as ``@AvoidChanges`` or ``@EnforceTranslation``, these will be
enforced to forbid some mutations.
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Contribute !
--------------
Contribute!
-----------
Golden Hinges is an open-source software originally written at the
`Edinburgh Genome Foundry <http://edinburgh-genome-foundry.github.io/home.html>`_
by `Zulko <https://github.com/Zulko>`_ and
`released on Github <https://github.com/Edinburgh-Genome-Foundry/GoldenHinges>`_
under the MIT licence. Everyone is welcome to contribute !
under the MIT licence. Everyone is welcome to contribute!
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