Skip to content

Commit

Permalink
PyPI readme
Browse files Browse the repository at this point in the history
  • Loading branch information
veghp committed Sep 11, 2020
1 parent 5afce58 commit 782ac94
Show file tree
Hide file tree
Showing 2 changed files with 77 additions and 1 deletion.
76 changes: 76 additions & 0 deletions pypi-readme.rst
@@ -0,0 +1,76 @@
BioPrinter
==========

.. image:: https://travis-ci.org/Edinburgh-Genome-Foundry/bioprinter.svg?branch=master
:target: https://travis-ci.org/Edinburgh-Genome-Foundry/bioprinter
:alt: Travis CI build status (Python 3)

.. image:: https://coveralls.io/repos/github/Edinburgh-Genome-Foundry/bioprinter/badge.svg?branch=master
:target: https://coveralls.io/github/Edinburgh-Genome-Foundry/bioprinter?branch=master


Bioprinter (webpage here_) is a Python package for producing living art. It transforms an image into files that a liquid dispenser can use to *print* the image to a plate using pigmented yeast or bacteria.

Here are two examples of bio-art:

.. figure:: https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/bioprinter/master/docs/_static/images/bioprint_dolly.jpeg
:align: center

Dolly drawn with baker yeast (white), violacein-producing yeast (black), and carotene-producing yeast (orange).


.. figure:: https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/bioprinter/master/docs/_static/images/bioprint_england.jpeg
:align: center

British flag drawn with 3 different strains of the bacterium *E. coli*.

These bio-prints (and many others!) were presented at the University of Edinburgh's SynthSys Open days 2016 (strain selection and acoustic printing by Paulina Kanigowska).

Bioprinter is released on Github_ under the MIT licence (Copyright 2015 Edinburgh Genome Foundry), everyone is welcome to contribute!

Bioprinter was written at the Edinburgh Genome Foundry by Zulko_ after an original idea and Matlab code by Mike Shen (`project on Github <https://github.com/mshen5/BioPointillism>`_).

Installation
------------

If you have PIP installed: ::

(sudo) pip install ez_setup bioprinter

Or unzip the source code in a directory and type in a terminal: ::

sudo python setup.py install


Usage
-----

The image file to be printed can have any resolution, but note that the width/height ratio of the plate the image is printed on is 1.5. Ensure that a specific color is used to mark the un-pigmented background of the image, here we use blue:

.. figure:: https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/bioprinter/master/docs/_static/images/dolly.jpeg
:align: center

Run the following code in Python or save in ``dolly.py``:

.. code:: python
from bioprinter import bioprint
bioprint(
image_filename="/path/to/image/dolly.jpeg",
output_filename="dolly.csv",
bg_color=[0, 0, 255], # blue background represents empty wells
pigments_wells={"A1": [0, 0, 0], # black yeast in source well A1
"A2": [250, 120, 10], # orange yeast in well A2
"A3": [255, 255, 255]}, # white yeast in well A3
quantified_image_filename="dolly_preview.jpeg"
)
The saved script can be executed in a terminal with ``python dolly.py``. This will produce a ``dolly.csv`` file as well as a preview image of the final printing (so that you can check if the image looks good at this low resolution).

Prepare a source plate with the right pigmented yeasts in wells A1, A2, A3, use an agar plate as the destination plate, and feed ``dolly.csv`` to the `Labcyte Echo Acoustic Liquid Handler <https://www.labcyte.com/products/liquid-handling/echo-liquid-handlers>`_ (tested with Labcyte Echo 555 Series). Once the printing is finished, incubate 2 days at 30C (it would be one day at 37C for bacteria). Enjoy the result!


.. _here: http://edinburgh-genome-foundry.github.io/bioprinter/
.. _Zulko: https://github.com/Zulko/
.. _Github: https://github.com/Edinburgh-Genome-Foundry/bioprinter
2 changes: 1 addition & 1 deletion setup.py
Expand Up @@ -11,7 +11,7 @@
version=__version__,
author="Zulko",
description="",
long_description=open("README.rst").read(),
long_description=open("pypi-readme.rst").read(),
license="MIT",
url="https://github.com/Edinburgh-Genome-Foundry/bioprinter",
keywords="bioprinter pointillism living art",
Expand Down

0 comments on commit 782ac94

Please sign in to comment.