Here it is a collection of processed datasets and code to reproduce most of the figures in our manuscript about DDX3 functions in HEK293 cells:

DDX3 depletion represses translation of mRNAs with complex 5′ UTRs

Lorenzo Calviello*, Srivats Venkataramanan*, Karol J Rogowski, Emanuel Wyler, Kevin Wilkins, Malvika Tejura, Bao Thai, Jacek Krol, Witold Filipowicz, Markus Landthaler^, Stephen N Floor^

biorXiv (2020), doi: https://doi.org/10.1101/589218

https://www.biorxiv.org/content/10.1101/589218v2

now peer-reviewed at NAR (2021), doi: https://doi.org/10.1093/nar/gkab287

https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab287/6255701

In this repository we included the post-processing analysis scripts for Ribo-seq, RNA-seq and PAR-CLIP experiments performed in HEK293 cells.

If you have any questions, please email:

calviello.l.bio@gmail.com (Lorenzo Calviello, data analysis), Stephen.floor@gmail.com (Stephen Floor, project supervisor), Markus.landthaler@mdc-berlin.de (Markus Landthaler, project supervisor).