In this project R version 3.6.0 was used.
The single-cell analysis was performed using the Seurat R-package. Versions for the various analysis parts can be found at the bottom of the analysis document.
Seurat single cell parameters, such as cluster-resolution and number of PCA dimensions used for UMAP dimensionality reduction can be found in the /design/sc.design.file.csv for each subanalysis.
Part 1: https://biologic.crick.ac.uk/SC20209B (access restricted to Tolar lab at present)
Part 2 (Subsetting based on gene filtering): https://biologic.crick.ac.uk/SC20209C (access restricted to Tolar lab at present)
Part 3 (Sub-subclustering): https://biologic.crick.ac.uk/SC20209D (access restricted to Tolar lab at present)