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readalignment.pb.go
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/
readalignment.pb.go
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// Code generated by protoc-gen-go. DO NOT EDIT.
// source: google/genomics/v1/readalignment.proto
package genomics
import proto "github.com/golang/protobuf/proto"
import fmt "fmt"
import math "math"
import _ "google.golang.org/genproto/googleapis/api/annotations"
import google_protobuf3 "github.com/golang/protobuf/ptypes/struct"
// Reference imports to suppress errors if they are not otherwise used.
var _ = proto.Marshal
var _ = fmt.Errorf
var _ = math.Inf
// A linear alignment can be represented by one CIGAR string. Describes the
// mapped position and local alignment of the read to the reference.
type LinearAlignment struct {
// The position of this alignment.
Position *Position `protobuf:"bytes,1,opt,name=position" json:"position,omitempty"`
// The mapping quality of this alignment. Represents how likely
// the read maps to this position as opposed to other locations.
//
// Specifically, this is -10 log10 Pr(mapping position is wrong), rounded to
// the nearest integer.
MappingQuality int32 `protobuf:"varint,2,opt,name=mapping_quality,json=mappingQuality" json:"mapping_quality,omitempty"`
// Represents the local alignment of this sequence (alignment matches, indels,
// etc) against the reference.
Cigar []*CigarUnit `protobuf:"bytes,3,rep,name=cigar" json:"cigar,omitempty"`
}
func (m *LinearAlignment) Reset() { *m = LinearAlignment{} }
func (m *LinearAlignment) String() string { return proto.CompactTextString(m) }
func (*LinearAlignment) ProtoMessage() {}
func (*LinearAlignment) Descriptor() ([]byte, []int) { return fileDescriptor6, []int{0} }
func (m *LinearAlignment) GetPosition() *Position {
if m != nil {
return m.Position
}
return nil
}
func (m *LinearAlignment) GetMappingQuality() int32 {
if m != nil {
return m.MappingQuality
}
return 0
}
func (m *LinearAlignment) GetCigar() []*CigarUnit {
if m != nil {
return m.Cigar
}
return nil
}
// A read alignment describes a linear alignment of a string of DNA to a
// [reference sequence][google.genomics.v1.Reference], in addition to metadata
// about the fragment (the molecule of DNA sequenced) and the read (the bases
// which were read by the sequencer). A read is equivalent to a line in a SAM
// file. A read belongs to exactly one read group and exactly one
// [read group set][google.genomics.v1.ReadGroupSet].
//
// For more genomics resource definitions, see [Fundamentals of Google
// Genomics](https://cloud.google.com/genomics/fundamentals-of-google-genomics)
//
// ### Reverse-stranded reads
//
// Mapped reads (reads having a non-null `alignment`) can be aligned to either
// the forward or the reverse strand of their associated reference. Strandedness
// of a mapped read is encoded by `alignment.position.reverseStrand`.
//
// If we consider the reference to be a forward-stranded coordinate space of
// `[0, reference.length)` with `0` as the left-most position and
// `reference.length` as the right-most position, reads are always aligned left
// to right. That is, `alignment.position.position` always refers to the
// left-most reference coordinate and `alignment.cigar` describes the alignment
// of this read to the reference from left to right. All per-base fields such as
// `alignedSequence` and `alignedQuality` share this same left-to-right
// orientation; this is true of reads which are aligned to either strand. For
// reverse-stranded reads, this means that `alignedSequence` is the reverse
// complement of the bases that were originally reported by the sequencing
// machine.
//
// ### Generating a reference-aligned sequence string
//
// When interacting with mapped reads, it's often useful to produce a string
// representing the local alignment of the read to reference. The following
// pseudocode demonstrates one way of doing this:
//
// out = ""
// offset = 0
// for c in read.alignment.cigar {
// switch c.operation {
// case "ALIGNMENT_MATCH", "SEQUENCE_MATCH", "SEQUENCE_MISMATCH":
// out += read.alignedSequence[offset:offset+c.operationLength]
// offset += c.operationLength
// break
// case "CLIP_SOFT", "INSERT":
// offset += c.operationLength
// break
// case "PAD":
// out += repeat("*", c.operationLength)
// break
// case "DELETE":
// out += repeat("-", c.operationLength)
// break
// case "SKIP":
// out += repeat(" ", c.operationLength)
// break
// case "CLIP_HARD":
// break
// }
// }
// return out
//
// ### Converting to SAM's CIGAR string
//
// The following pseudocode generates a SAM CIGAR string from the
// `cigar` field. Note that this is a lossy conversion
// (`cigar.referenceSequence` is lost).
//
// cigarMap = {
// "ALIGNMENT_MATCH": "M",
// "INSERT": "I",
// "DELETE": "D",
// "SKIP": "N",
// "CLIP_SOFT": "S",
// "CLIP_HARD": "H",
// "PAD": "P",
// "SEQUENCE_MATCH": "=",
// "SEQUENCE_MISMATCH": "X",
// }
// cigarStr = ""
// for c in read.alignment.cigar {
// cigarStr += c.operationLength + cigarMap[c.operation]
// }
// return cigarStr
type Read struct {
// The server-generated read ID, unique across all reads. This is different
// from the `fragmentName`.
Id string `protobuf:"bytes,1,opt,name=id" json:"id,omitempty"`
// The ID of the read group this read belongs to. A read belongs to exactly
// one read group. This is a server-generated ID which is distinct from SAM's
// RG tag (for that value, see
// [ReadGroup.name][google.genomics.v1.ReadGroup.name]).
ReadGroupId string `protobuf:"bytes,2,opt,name=read_group_id,json=readGroupId" json:"read_group_id,omitempty"`
// The ID of the read group set this read belongs to. A read belongs to
// exactly one read group set.
ReadGroupSetId string `protobuf:"bytes,3,opt,name=read_group_set_id,json=readGroupSetId" json:"read_group_set_id,omitempty"`
// The fragment name. Equivalent to QNAME (query template name) in SAM.
FragmentName string `protobuf:"bytes,4,opt,name=fragment_name,json=fragmentName" json:"fragment_name,omitempty"`
// The orientation and the distance between reads from the fragment are
// consistent with the sequencing protocol (SAM flag 0x2).
ProperPlacement bool `protobuf:"varint,5,opt,name=proper_placement,json=properPlacement" json:"proper_placement,omitempty"`
// The fragment is a PCR or optical duplicate (SAM flag 0x400).
DuplicateFragment bool `protobuf:"varint,6,opt,name=duplicate_fragment,json=duplicateFragment" json:"duplicate_fragment,omitempty"`
// The observed length of the fragment, equivalent to TLEN in SAM.
FragmentLength int32 `protobuf:"varint,7,opt,name=fragment_length,json=fragmentLength" json:"fragment_length,omitempty"`
// The read number in sequencing. 0-based and less than numberReads. This
// field replaces SAM flag 0x40 and 0x80.
ReadNumber int32 `protobuf:"varint,8,opt,name=read_number,json=readNumber" json:"read_number,omitempty"`
// The number of reads in the fragment (extension to SAM flag 0x1).
NumberReads int32 `protobuf:"varint,9,opt,name=number_reads,json=numberReads" json:"number_reads,omitempty"`
// Whether this read did not pass filters, such as platform or vendor quality
// controls (SAM flag 0x200).
FailedVendorQualityChecks bool `protobuf:"varint,10,opt,name=failed_vendor_quality_checks,json=failedVendorQualityChecks" json:"failed_vendor_quality_checks,omitempty"`
// The linear alignment for this alignment record. This field is null for
// unmapped reads.
Alignment *LinearAlignment `protobuf:"bytes,11,opt,name=alignment" json:"alignment,omitempty"`
// Whether this alignment is secondary. Equivalent to SAM flag 0x100.
// A secondary alignment represents an alternative to the primary alignment
// for this read. Aligners may return secondary alignments if a read can map
// ambiguously to multiple coordinates in the genome. By convention, each read
// has one and only one alignment where both `secondaryAlignment`
// and `supplementaryAlignment` are false.
SecondaryAlignment bool `protobuf:"varint,12,opt,name=secondary_alignment,json=secondaryAlignment" json:"secondary_alignment,omitempty"`
// Whether this alignment is supplementary. Equivalent to SAM flag 0x800.
// Supplementary alignments are used in the representation of a chimeric
// alignment. In a chimeric alignment, a read is split into multiple
// linear alignments that map to different reference contigs. The first
// linear alignment in the read will be designated as the representative
// alignment; the remaining linear alignments will be designated as
// supplementary alignments. These alignments may have different mapping
// quality scores. In each linear alignment in a chimeric alignment, the read
// will be hard clipped. The `alignedSequence` and
// `alignedQuality` fields in the alignment record will only
// represent the bases for its respective linear alignment.
SupplementaryAlignment bool `protobuf:"varint,13,opt,name=supplementary_alignment,json=supplementaryAlignment" json:"supplementary_alignment,omitempty"`
// The bases of the read sequence contained in this alignment record,
// **without CIGAR operations applied** (equivalent to SEQ in SAM).
// `alignedSequence` and `alignedQuality` may be
// shorter than the full read sequence and quality. This will occur if the
// alignment is part of a chimeric alignment, or if the read was trimmed. When
// this occurs, the CIGAR for this read will begin/end with a hard clip
// operator that will indicate the length of the excised sequence.
AlignedSequence string `protobuf:"bytes,14,opt,name=aligned_sequence,json=alignedSequence" json:"aligned_sequence,omitempty"`
// The quality of the read sequence contained in this alignment record
// (equivalent to QUAL in SAM).
// `alignedSequence` and `alignedQuality` may be shorter than the full read
// sequence and quality. This will occur if the alignment is part of a
// chimeric alignment, or if the read was trimmed. When this occurs, the CIGAR
// for this read will begin/end with a hard clip operator that will indicate
// the length of the excised sequence.
AlignedQuality []int32 `protobuf:"varint,15,rep,packed,name=aligned_quality,json=alignedQuality" json:"aligned_quality,omitempty"`
// The mapping of the primary alignment of the
// `(readNumber+1)%numberReads` read in the fragment. It replaces
// mate position and mate strand in SAM.
NextMatePosition *Position `protobuf:"bytes,16,opt,name=next_mate_position,json=nextMatePosition" json:"next_mate_position,omitempty"`
// A map of additional read alignment information. This must be of the form
// map<string, string[]> (string key mapping to a list of string values).
Info map[string]*google_protobuf3.ListValue `protobuf:"bytes,17,rep,name=info" json:"info,omitempty" protobuf_key:"bytes,1,opt,name=key" protobuf_val:"bytes,2,opt,name=value"`
}
func (m *Read) Reset() { *m = Read{} }
func (m *Read) String() string { return proto.CompactTextString(m) }
func (*Read) ProtoMessage() {}
func (*Read) Descriptor() ([]byte, []int) { return fileDescriptor6, []int{1} }
func (m *Read) GetId() string {
if m != nil {
return m.Id
}
return ""
}
func (m *Read) GetReadGroupId() string {
if m != nil {
return m.ReadGroupId
}
return ""
}
func (m *Read) GetReadGroupSetId() string {
if m != nil {
return m.ReadGroupSetId
}
return ""
}
func (m *Read) GetFragmentName() string {
if m != nil {
return m.FragmentName
}
return ""
}
func (m *Read) GetProperPlacement() bool {
if m != nil {
return m.ProperPlacement
}
return false
}
func (m *Read) GetDuplicateFragment() bool {
if m != nil {
return m.DuplicateFragment
}
return false
}
func (m *Read) GetFragmentLength() int32 {
if m != nil {
return m.FragmentLength
}
return 0
}
func (m *Read) GetReadNumber() int32 {
if m != nil {
return m.ReadNumber
}
return 0
}
func (m *Read) GetNumberReads() int32 {
if m != nil {
return m.NumberReads
}
return 0
}
func (m *Read) GetFailedVendorQualityChecks() bool {
if m != nil {
return m.FailedVendorQualityChecks
}
return false
}
func (m *Read) GetAlignment() *LinearAlignment {
if m != nil {
return m.Alignment
}
return nil
}
func (m *Read) GetSecondaryAlignment() bool {
if m != nil {
return m.SecondaryAlignment
}
return false
}
func (m *Read) GetSupplementaryAlignment() bool {
if m != nil {
return m.SupplementaryAlignment
}
return false
}
func (m *Read) GetAlignedSequence() string {
if m != nil {
return m.AlignedSequence
}
return ""
}
func (m *Read) GetAlignedQuality() []int32 {
if m != nil {
return m.AlignedQuality
}
return nil
}
func (m *Read) GetNextMatePosition() *Position {
if m != nil {
return m.NextMatePosition
}
return nil
}
func (m *Read) GetInfo() map[string]*google_protobuf3.ListValue {
if m != nil {
return m.Info
}
return nil
}
func init() {
proto.RegisterType((*LinearAlignment)(nil), "google.genomics.v1.LinearAlignment")
proto.RegisterType((*Read)(nil), "google.genomics.v1.Read")
}
func init() { proto.RegisterFile("google/genomics/v1/readalignment.proto", fileDescriptor6) }
var fileDescriptor6 = []byte{
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