The CRTC Toolkit provides a series of applications which allow for annotating and collecting individual cells from microscopy images, segmenting those cells into nuclear and cytoplasmic regions, and performing data analysis using the relative expression levels of CRTC in each region. The toolkit is provided as a set of compiled MATLAB apps, with source code available for modification and further scripting of the pipeline and workflows.
Usage
For the MacOS toolkit, the ResNet must be downloaded and used in the Segment Cells portion of the workflow independently. For the Windows version, the ResNet should be installed and used automatically.