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<p> | ||
This is a two genetic marker phylogenetic tree: hybrid tree | ||
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Installation instructions:<p> | ||
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For BOTH scripts, you need 3 input files:<p> | ||
1)repset of ITS seqs<p> | ||
2)taxonomy file<p> | ||
--above are in Google Drive (will share)<p> | ||
3)Silva 18S file:<p> | ||
http://www.arb-silva.de/fileadmin/silva_databases/release_115/Exports/SSURef_NR99_115_tax_silva_full_align_trunc.fasta.tgz | ||
(very large, full alignment, non-redundant SSU, has ALL eukaryotes which is unnecessary) | ||
<p> | ||
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There are two scripts, one relies on Qiime (make_hybrid_tree.py) and the other one (skbio_hybrid_tree_wip.py) uses skbio with FastTree and Muscle installed locally | ||
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"python make_hybrid_tree.py" can be run in command line in QIIME in the working directory where your 3 files are; It uses Muscle and Fasttree from within QIIME (and other Qiime scripts) | ||
<p> | ||
<p> | ||
skbio_hybrid_tree_wip.py is the transition away from Qiime dependency; CHANGE input files at the bottom of python script & change directory of FastTree and Muscle (you must download and install locally) | ||
<p> | ||
<p> |