Skip to content

Commit

Permalink
Merge 51b13dd into f0ab25a
Browse files Browse the repository at this point in the history
  • Loading branch information
JTFouquier committed Feb 6, 2015
2 parents f0ab25a + 51b13dd commit f881e34
Show file tree
Hide file tree
Showing 2 changed files with 30 additions and 24 deletions.
32 changes: 8 additions & 24 deletions README.rst
Original file line number Diff line number Diff line change
@@ -1,31 +1,15 @@
hybrid_tree
===========
ghost-tree
==========

|Build Status| |Coverage Status|

<p>
This is a two genetic marker phylogenetic tree: hybrid tree

Installation instructions:<p>

For BOTH scripts, you need 3 input files:<p>
1)repset of ITS seqs<p>
2)taxonomy file<p>
--above are in Google Drive (will share)<p>
3)Silva 18S file:<p>
http://www.arb-silva.de/fileadmin/silva_databases/release_115/Exports/SSURef_NR99_115_tax_silva_full_align_trunc.fasta.tgz
(very large, full alignment, non-redundant SSU, has ALL eukaryotes which is unnecessary)
<p>

There are two scripts, one relies on Qiime (make_hybrid_tree.py) and the other one (skbio_hybrid_tree_wip.py) uses skbio with FastTree and Muscle installed locally

"python make_hybrid_tree.py" can be run in command line in QIIME in the working directory where your 3 files are; It uses Muscle and Fasttree from within QIIME (and other Qiime scripts)
<p>
<p>
skbio_hybrid_tree_wip.py is the transition away from Qiime dependency; CHANGE input files at the bottom of python script & change directory of FastTree and Muscle (you must download and install locally)
<p>
<p>
ghost-tree is a bioinformatics tool that combines sequence data from two
genetic marker databases into one phylogenetic tree that can be used for
diversity analyses. One database is used as a backbone or scaffold because it
provides better phylogeny across all phyla, and the other database provides
finer taxonomic resolution.

This project is currently under active development.


.. |Build Status| image:: https://travis-ci.org/JTFouquier/ghost-tree.svg?branch=master
Expand Down
22 changes: 22 additions & 0 deletions legacy/README.html
Original file line number Diff line number Diff line change
@@ -0,0 +1,22 @@
<p>
This is a two genetic marker phylogenetic tree: hybrid tree

Installation instructions:<p>

For BOTH scripts, you need 3 input files:<p>
1)repset of ITS seqs<p>
2)taxonomy file<p>
--above are in Google Drive (will share)<p>
3)Silva 18S file:<p>
http://www.arb-silva.de/fileadmin/silva_databases/release_115/Exports/SSURef_NR99_115_tax_silva_full_align_trunc.fasta.tgz
(very large, full alignment, non-redundant SSU, has ALL eukaryotes which is unnecessary)
<p>

There are two scripts, one relies on Qiime (make_hybrid_tree.py) and the other one (skbio_hybrid_tree_wip.py) uses skbio with FastTree and Muscle installed locally

"python make_hybrid_tree.py" can be run in command line in QIIME in the working directory where your 3 files are; It uses Muscle and Fasttree from within QIIME (and other Qiime scripts)
<p>
<p>
skbio_hybrid_tree_wip.py is the transition away from Qiime dependency; CHANGE input files at the bottom of python script & change directory of FastTree and Muscle (you must download and install locally)
<p>
<p>

0 comments on commit f881e34

Please sign in to comment.