Metabarcoding Pipeline

This pipeline was used to process and analyse metabarcoding data for the publication:

Stuart, J., et al., A comparison of two gene regions for assessing community composition of eukaryotic marine microalgae from coastal ecosystems. Scientific Reports, 2024. 14(1): p. 6442. doi: 10.1038/s41598-024-56993-4

Data types and file layout

There were three main types of input files for the analysis in this pipeline:

1. Metabarcoding files
2. Taxonomy files
3. Metadata

Metadata layout

Sample/sequence metadata files were in the format depicted below