Part files and drawings for the mouse head-bars/holders used during IOS & 2P Imaging
https://kl-turner.github.io/Mouse-Head-Fixation/
This repository contains numerous SolidWorks part files, drawing files, and PDFs of those drawing files for numerous head-bar sizes, as well some of the different holders for those head-bars used during imaging. The head-bars are implanted during surgery (See https://www.nature.com/articles/s41593-017-0007-y and https://www.jove.com/video/3742/a-polished-reinforced-thinned-skull-window-for-long-term-imaging for more details) with the headbar being secured in the holder during data acquisition.
- Adult head-bar 0.035 inches thick
- Adult head-bar 0.050 inches thick
- Adult head-bar 0.063 inches thick
- Adult head-bar 0.035 inches thick with a center shank
- Adult head-bar 0.050 inches thick with a center shank
- Adult head-bar 0.063 inches thick with a center shank
- Adult head-bar 0.050 inches thick with a 0.025 inch cut out of the center
- Surgical head-bar holder
- Running wheel head-bar holder
- Tube (rest/sleep imaging) head-bar holder
For quick inspection, simply download the PDF of the desired part. The following images include just a few of the part files, as well as some images of them after fabrication.
| Sample Head-bar | Running wheel holder | Resting imaging holder |
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| Sample Head-bar | Resting imaging holder |
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The head-bars are CNC machined from a Titanium sheet (Commerically Pure [CP] Grade 2) of desired thickness due to its biocompatibility, anti-corrosion properties, and overall strength. Titanium is also resistant to acetone, which is used to dissolve the various adhesives to secure the head-bar, thinned-skull windows, electrodes, cannula, and other components during surgery. These bars are designed to be cleaned and re-used indefinitely.
Headbar holders are CNC machined from an Aluminum sheet (6061-T6511) of desired thickness due to it being easily machinable, light-weight, anti-corrosion properties, and having sufficient strength (there's no significant stresses applied to any part) for the desired applications.
If any of the individual part files have a listed material, disregard it in favor of the aforementioned
| Running wheel holder | Resting imaging holder |
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| Resting imaging holder in a two-photon imaging set-up |
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| Resting imaging holder in an IOS imaging set-up |
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The various part files were originally designed and repeatedly revisioned by numerous members of the Drew Lab (Including, but certainly not limited to:)
- Gar Waterman
- Yurong Guo
- Bingxing Huo
- Aaron Winder
- Kyle Gheres
- Qingguang Zhang
- Kevin Turner
This is only a small sample set of the head-bars and holders used in the Drew Lab. If you are interested in other specific files, such as those used during neonate experiments or others not mentioned, contact klt8@psu.edu.