Single-cell analysis of PBMC from MPA patients and Healthy donors

Overview

This repository provides code used for CITE-seq analyses of >100,000 PBMCs from 8 MPA (Mocroscopic Polyangiitis) patients and 7 healthy donors.

Nishide M, Nishimura K, et al, "Single-cell multi-omics analysis identifies two distinct phenotypes of newly-onset microscopic polyangiitis", submitted.

Software requirements

・R version 4.1.0 ・Seurat (v4.2.0) ・miloR (v3.15)

Data availability

Expression matrix files are available at the Japanese Genotype-phenotype Archive (JGA) with accession codes [JGA000XXX] (https://ddbj.nig.ac.jp/resource/jga-study/JGAS000XXX)