Drop-seq-PjHem

These are commads for Drop-seq analyzing of hemocytes of kuruma shrimp (Penaues japonicus).

The detailed scientific explanation is published under BioRxiv.
Here, I uploaded commads used on this paper.

The data have been deposited with links to BioProject accession number PRJDB15011 in the DDBJ BioProject database.

Command lines for Drop-seq analysis of shrimp hemocytes

command link

The raw sequence data used in this study were archived in DDBJ Sequence Read Archive (DRA) of the DNA Data Bank of Japan: Drop-seq male shrimp (uninfected) rep1: DRR426776, male shrimp (uninfected) rep2: DRR426777, male shrimp (infected) rep1: DRR426778, male shrimp (infected) rep2: DRR426779, female shrimp (uninfected) rep1: DRR426780, female shrimp (uninfected) rep2: DRR426781, female shrimp (infected) rep1: DRR426782, female shrimp (infected) rep2: DRR426783, under DRA015407.

Command lines for Seurat

Data filtration, UMAP reductuion, clustering and marker findings by Seurat. command link