KisleyLabAtCWRU/Peroxisome-iFCCS
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%% PeroxisomeCorrelationMain_LK20221006.m % image analysis to perform image correlation on mCherry and GFP-labeled % peroxisome proteins % Analysis includes sections to: % 1. Load data (read comments in this section about the input data format) % 2. Image registration of GFP and mCherry channels based on control point selection, % correlation, and image transformation using fiducial bead markers % 3. Find location of peroxisomes using existing particle tracking code % (requires Troika single particle tracking code maintained by Prof. % Christy Landes' group available at: % https://github.com/LandesLab/Troika-Single-particle-tracking; relevant % citation is DOI: 10.1039/C3CP53968G % 4. For each peroxisome, perform image fluorscence cross correlation % spectroscopy at the 17 pixels surrounding the centroid location (read % comments in this section regarding format of the data saved) % 5. Save data (optional) % 6. Make figures of results (optional) % Contact Prof. Lydia Kisley, Case Western Reserve University, % lydia.kisley@case.edu for any questions
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Script for imaging fluorescence cross correlation analysis for "Peroxisome biogenesis initiated by protein phase separation"
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