Skip to content

v0.5.3

Compare
Choose a tag to compare
@jjti jjti released this 15 Nov 03:29
· 7 commits to master since this release

Full Changelog: v0.5.0...v0.5.3

  • primers score SEQ1 SEQ2 -s TEMPLATE_SEQ now returns separate tm and tm_totals. This affects score:
$ primers score GGTCTCAATGAGACAATA AAAAAATTTCGTATGCTGACCTAG -s AATGAGACAATAGCACACACAGCTAGGTCAGCATACGAAA --json | jq
[
  {
    "seq": "GGTCTCAATGAGACAATA",
    "len": 18,
    "tm": 39.4,
    "tm_total": 55,
    "gc": 0.4,
    "dg": -1.86,
    "fwd": true,
    "off_target_count": 0,
    "scoring": {
      "penalty": 49.9,
      "penalty_tm": 22.6,
      "penalty_tm_diff": 19.6,
      "penalty_gc": 2,
      "penalty_len": 2,
      "penalty_dg": 3.7,
      "penalty_off_target": 0
    }
  },
  {
    "seq": "AAAAAATTTCGTATGCTGACCTAG",
    "len": 24,
    "tm": 59,
    "tm_total": 61.6,
    "gc": 0.4,
    "dg": 0,
    "fwd": false,
    "off_target_count": 0,
    "scoring": {
      "penalty": 25.6,
      "penalty_tm": 3,
      "penalty_tm_diff": 19.6,
      "penalty_gc": 2,
      "penalty_len": 1,
      "penalty_dg": 0,
      "penalty_off_target": 0
    }
  }
]