Motion Analysis of Ring-Shaped Tissues v1.0

For licensing, please see LICENSE.txt

If you use this software in a scientific publication, please cite:

Seguret Magali, Davidson Patricia, Robben Stijn, Jouve Charlène, Pereira Céline, Cerveau Cyril, Le Berre Maël, Rodrigues Ribeiro Rita S., Hulot Jean-Sébastien (2023) A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell derived cardiomyocytes eLife 12:RP87739, https://doi.org/10.7554/eLife.87739.1.

Purpose and scope

This code allows the contractility analysis of ring-shaped cardiac tissues by the evaluation of the area of the inner circle defined by the tissue in time.

Summary of main files

MotionAnalysis.m: main code, loads videos, generates figures and creates an Excel file containing the contractility parameters of all the videos and a .mat file of all the videos.

Requirements

MATLAB MATLAB Image Processing Toolbox MATLAB Signal Processing Toolbox

Example video

An example video can be found in the article to test the code before use.