Förster Resonance Energy Transfer Calculator (FRET-Calc) is a user-friendly software that analyzes experimental data for molar extinction coefficient of the acceptor, emission spectrum of the donor and the refractive index spectrum of the donor/acceptor blend. Therefore, the primary objective of this software is to acquire the fundamental parameters involved in the FRET process, such as:

• Effective refractive index
• Overlap integral
• Förster radius
• FRET efficiency
• FRET rate

Detailed instructions for using FRET-Calc can be consulted in our published article:

FRET−Calc: A Free Software and Web Server for Förster Resonance Energy Transfer Calculation, Computer Physics Communications, 287, 2023, 108715.

The preprint of this article is available for download: here.

The software binaries can be downloaded for the following operating systems: Unix-like systems (Linux), Windows and macOS.

Program interface:

FRET-Calc can also be used online on our website.

Test the app using sample data.

More spectral data for testing the app can be downloaded here.

Find out more about our research groups: DiNE and NAMOR.

Authors

• Leandro Benatto^a,b
• Omar Mesquita^a
• João L. B. Rosa^b
• Lucimara S. Roman^b
• Marlus Koehler^b
• Rodrigo B. Capaz^a,c
• Graziâni Candiotto^a

Institutions

^aInstitute of Physics, Federal University of Rio de Janeiro, 21941-909, Rio de Janeiro-RJ, Brazil.
UFRJ

^bDepartment of Physics, Federal University of Paraná, 81531-980, Curitiba-PR, Brazil.
UFPR

^cBrazilian Nanotechnology National Laboratory (LNNano), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-100, Campinas- SP, Brazil.
LNNano

Developers

• Leandro Benatto
• Omar Mesquita
• Graziâni Candiotto

Data Sample

• The data-sample folder contains an example of the data set needed to run FRETCalc.
  You can download them here

Spectral Data

In the spectral-data folder, you will find additional data that can be used to obtain FRET parameters for different molecules. Which are:

• Emission spectrum of the donor
• Extinction coefficient spectrum of the acceptor
• Refractive index spectrum of the donor/acceptor blend

You can also directly download them here

Acknowledgments

The authors acknowledge financial support from CNPq (grant 381113/2021-3) and LCNano/SisNANO 2.0 (grant 442591/2019-5). L.B. (grant E-26/202.091/2022 process 277806), O.M. (grant E-26/200.729/2023 process 285493) and G.C. (grant E-26/200.627/2022 and E-26/210.391/2022 process 271814) are greatfully for financial support from FAPERJ. The authors also acknowledge the computational support of Núcleo Avançado de Computação de Alto Desempenho (NACAD/COPPE/UFRJ), Sistema Nacional de Processamento de Alto Desempenho (SINAPAD) and Centro Nacional de Processamento de Alto Desempenho em São Paulo (CENAPAD-SP) and technical support of SMMOL - solutions in functionalyzed materials.

How to cite FRET-Calc

If you use FRET-Calc for your data analysis, please indicate in your published work by citing:

• FRET−Calc: A Free Software and Web Server for Förster Resonance Energy Transfer Calculation, Comput. Phys. Commun., 287, 2023, 108715.

Cited by

Papers that recently cited FRET-Calc are shown below.

[1] Improved Performance of Organic Light-Emitting Transistors Enabled by Polyurethane Gate Dielectric, ACS Appl. Mater. Interfaces, 2023, 15, 28, 33809–33818.

[2] Methods for Roll-to-Roll Vapor Deposition of Organic Semiconductors, UNIVERSITY OF MICHIGAN, Thesis, 2023.

[3] Synergistic Influence of FRET on a High Quantum Yield π-Conjugate Polycarbazole-ZnS Composite Thin Film, ACS Omega, 2023.

[4] PLQ−sim: A computational tool for simulating photoluminescence quenching dynamics in organic donor/acceptor blends, Comput. Phys. Commun., Volume 296, March 2024, 109015.

[5] RI−Calc: A user friendly software and web server for refractive index calculation, Comput. Phys. Commun., Volume 298, May 2024, 109100.

[6] Design and characterization of BSA-mycophenolic acid nanocomplexes: Antiviral activity exploration, International Journal of Biological Macromolecules, Volume 265, Part 2, April 2024, 131023.