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Includes GUI and 3D pose estimation pipeline for tethered Drosophila.
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License: GPL v3

Alt text

DeepFly3D is a PyTorch and PyQT5 implementation of 2D-3D tethered Drosophila pose estimation. It aims to provide an interface for pose estimation and to permit further correction of 2D pose estimates, which are automatically converted to 3D pose.

DeepFly3D does not require a calibration pattern, it enforces geometric constraints using pictorial structures, which corrects most of the erros, and the remaining errors are automatically detected can be dealt easily with GUI assistance.

Code for data preparation and augmentation are taken from the Stacked hourglass network. We also use the Stacked Hourglass model for 2D pose estimation. We implement custom advances (e.g., GUI) using PyQT5.


Alt text DeepFly3D provides a nice GUI to interact with the data. Using DeepFly3D GUI, you can visualize:

  • Raw data
  • Probability maps
  • Raw predictions
  • Automatic corrections

And you can perform:

  • 2D pose estimation
  • Calibration without calibration pattern
  • Saving the final 3D estimations
  • Manual Correction

Identifying erroneous estimates automatically

Alt text DeepFly3D can automatically detect when 2D pose estimation is failed.


Alt text DeepFly3D will try to fix these mistaked using multi-view geometry and pictorial structures. In the next iteration of training, you can also use these examples to train the 2D estimation network! Auto-correction is perfomed if 2D pose estimation and calibration are complete.

Assisting manual correction

In the 'Correction' mode, the GUI tries to correct errors using pictorial structures. To save these corrections, press T. Please check the associated manuscript (Günel et al. 2019) for implementation details. Alt text In the next iteration of training, you can use these examples to train the network!


And nice visualizations! Check the Visualization doc for details. In general, displaying pose estimation results should be as easy as:

import matplotlib.pyplot as plt
from deepfly.GUI.CameraNetwork import CameraNetwork
camNet = CameraNetwork(image_folder=image_folder)
image_folder = './data/test'


and to display heatmaps:


To create more complicated figures, or replicate the figures from the paper, you can use the the pose_result file which is saved in the same folder as the images. The notebook, notebook_visualize/visualize.ipynb, shows you the steps to create the following figure:

To visualize the time series instead, use the notebook notebook_visualize/time_series.ipynb. It should output 2D/3D pose, along with a few selected time series.

Using different datasets

It is possible to use DeepFly3D on other animals! Check the Adaptation text to see an example of H3.6m multi-view human dataset.


  author    = {Semih Gunel and
               Helge Rhodin and
               Daniel Morales and
               João Compagnolo and
               Pavan Ramdya and
               Pascal Fua},
  title     = {DeepFly3D: A deep learning-based approach for 3D limb and appendage tracking in tethered, adult Drosophila},
  bookTitle = {bioRxiv}
  year      = {2019}

  author    = {Alejandro Newell and
               Kaiyu Yang and
               Jia Deng},
  title     = {Stacked Hourglass Networks for Human Pose Estimation},
  booktitle = {Computer Vision - {ECCV} 2016 - 14th European Conference, Amsterdam,
               The Netherlands, October 11-14, 2016, Proceedings, Part {VIII}},
  pages     = {483--499},
  year      = {2016},
  doi       = {10.1007/978-3-319-46484-8\_29},

We want to thank to Florian Aymanns for testing of the software and for his helpful comments.

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