Iba1+ cells in the PEC are generally sparsely distributed around the posterior eye cup, but manual quantification of these cells is time consuming. Therefore we developed a second semi-automated MatLab code to quantify single Iba1+ cells. The operator is presented with masked, randomized images and allowed to remove non-specific fluorescence (fa…

Confluent and overlapping cells are difficult to quantify in low power images. This MatLab code allows a user to analyze the pixel intensity (integrated density) over a region of interest (ROI) in low power images as a surrogate for cell number. This code was optimized for use in our Choroidal Neovascularization model, and allows for the analysi…