Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Using empty placeholder bam files to process a large number of files #430

Closed
ontalin opened this issue Nov 25, 2020 · 1 comment
Closed

Comments

@ontalin
Copy link

ontalin commented Nov 25, 2020

In order to jointly call SVs from a large number of samples, I followed the pipeline in #354 to create empty placeholder bam files and preprocessed them for batch assembly. However, when preprocessing the empty bam files, which have only the headers, gridss unsurprisingly failed to produce the EMPTY_BAM.insert_size_metrics and gave the following error message:

Wed Nov 25 16:50:47 CST 2020   CollectGridssMetricsAndExtractSVReads|samtools	EMPTY_BAM
rm: cannot remove './EMPTY_BAM.bam.gridss.working/tmp.empty_AF04.bam.insert_size_metrics': No such file or directory
rm: cannot remove './EMPTY_BAM.bam.gridss.working/tmp.empty_AF04.bam.insert_size_histogram.pdf': No such file or directory

Three files required for following assembly: EMPTY_BAM.insert_size_metrics, EMPTY_BAM.sv.bam, and EMPTY_BAM.sv.bam.bai are absent as well.

Is there a solution to this pipeline?

Thank you in advance.

@d-cameron
Copy link
Member

Work-around no longer required :)

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants