GlyCombo is an open source software for combinatorial glycan composition determination to identify glycans in MS acquisitions of glycan-containing samples in text or mzML formats. This application enables rapid extraction of precursor m/z values from mzML files, a vendor-neutral format that ensures cross-platform compatibility.
- Open-source Windows application created in C#.
- Extensive monosaccharide and modification options including Hex, HexNAc, dHex, Neu5Ac, Neu5Gc, HexN, HexA, dHexNAc, Pent, KDN, Phosphate and Sulfate.
- Support for native and permethylated glycan samples.
- Support for free and reduced glycans.
- Automatic polarity extraction for mzML input files.
| Output Options | mzML File Input | Text List of Masses |
|---|---|---|
| CSV file of glycan combinations | ✔️ | ✔️ |
| Parameters File | ✔️ | ✔️ |
| Skyline Import File | ✔️ |
Please cite:
Kelly MI, Ashwood C. GlyCombo enables rapid, complete glycan composition identification across diverse glycomic sample types. ChemRxiv. 2024; doi:10.26434/chemrxiv-2024-3j008 This content is a preprint and has not been peer-reviewed.
GlyCombo is released under the Apache 2.0 License.