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Update quickguide.rst
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Olivier Gueudelot committed Mar 13, 2017
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16 changes: 14 additions & 2 deletions docs/quickguide.rst
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Expand Up @@ -94,10 +94,22 @@ Below the command to get all files for hg19 from the gatk public repository ::
nohup wget ftp://gsapubftp-anonymous@ftp.broadinstitute.org/bundle/hg19/ -r &
# nohup allow the long task to run without bocking your shell session
# you can follow the execution by looking the log
tail -f nohup.out
# when all download are completed
tail -f nohup.out # Ctrl+C to quit
# meanwhile, downloading hg19.fa ref
wget http://hgdownload.soe.ucsc.edu/goldenPath/hg19/bigZips/hg19.2bit
md5sum hg19.2bit # compare fingerprint with online md5
# TODO : choice 1 : installing bioinfo tools on the server or choice 2 : download all file from a directory ?
twoBitToFa hg19.2bit hg19.fa
samtools faidx hg19.fa
bwa index hg19.fa
# when all other downloads are completed (look into nohup.out file)
mv ftp.broadinstitute.org/bundle/hg19/* .
# Check that file are not corrupted
cat *.md5 >> all.md5
sed -i 's/humgen\/gsa-scr1\/pub\/bundle\/2.8\/hg19/var\/pirus\/databases\/hg19/' all.md5
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