BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event

Data and analysis files from a study using BONCAT-FACS-Seq to identify active microbes in a biocrust microbial community

Trexler RV, Van Goethem MW, Goudeau D, Nath N, Malmstrom RR, Northen TR, Couradeau E (2023) BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event. Front. Microbiol., 14:1176751. https://doi.org/10.3389/fmicb.2023.1176751

Files

R analyses

This directory contains all R based analyses

• Bracken_Analysis.R: analysis of taxonomic data from metagenomes produced via BONCAT-FACS-Seq.
• COG_Analysis.R: analysis of COG data extracted from IMG/M of the metagenomes produced via BONCAT-FACS-Seq.

Data

This directory contains important data not included in the manuscript supplementary material. The raw sequencing data can be found on the NCBI SRA under BioProject PRJNA938738.

• Metadata_key.txt: metadata table describing samples; used in both Bracken_Analysis.R and COG_Analysis.R.
• bracken_combined_v2.txt: taxa table output from bracken; analyzed in Bracken_Analysis.R.
• COG_Data.zip: zipped directory containing the COG data from each metagenome sample; analyzed in COG_Analysis.R.
• COG_ID_Annotation.txt: data table linking COG IDs to associated annotation data; used in COG_Analysis.R.
• COG_Categories_Expanded.txt: data table COG Category IDs to associated annotation data; used in COG_Analysis.R.