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Clarify that taxinfo is available after rfmake, not rfsearch
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AntonPetrov committed Oct 24, 2019
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11 changes: 8 additions & 3 deletions docs/source/rfam-cloud.rst
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Expand Up @@ -99,7 +99,7 @@ Build and calibrate a :ref:`glossary:Covariance model (CM)` based on your seed a
4. Choose a gathering threshold
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

The output files (``species``, ``outlist``, and ``taxinfo``) should be used to determine the gathering threshold for this family (the bit score of the last true positive hit).
The output files (:ref:`choosing-gathering-threshold:Species file` and :ref:`choosing-gathering-threshold:Outlist file`) can be used to determine the gathering threshold for this family (the bit score of the last true positive hit).

.. NOTE::
For detailed instructions on how to select the threshold, see :ref:`choosing-gathering-threshold:Choosing gathering threshold`.
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rfmake.pl -t gathering_cutoff -a

For more information about setting the ``-t`` parameter, see :ref:`choosing-gathering-threshold:Choosing gathering threshold`.
The ``-a`` option creates an ``align`` file with an alignment of all the sequences above the gathering threshold. For more information about setting the ``-t`` parameter, see :ref:`choosing-gathering-threshold:Choosing gathering threshold`.

The ``-a`` option creates an ``align`` file with an alignment of all the sequences above the gathering threshold. Reviewing the ``align`` file can help to adjust the threshold, as the unwanted sequences can be excluded by rerunning rfmake with a higher threshold ``-t``.
After running rfmake you should:

- review the :ref:`choosing-gathering-threshold:Align file` to check that the threshold is set correctly.
- review the :ref:`choosing-gathering-threshold:Taxinfo file` to check that the taxonomic distribution of the family is correct.

Any unwanted sequences can be excluded by rerunning rfmake with a higher threshold ``-t``.

8. Add metadata to the DESC file
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
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