tic_tac_toe_game.py

from tkinter import *

def callback(r, c):
    global player
    if player == 'X' and states[r][c] == 0 and not stop_game:
        b[r][c].configure(text='X', bg="white")
        states[r][c] = 'X'
        player = 'O'
    elif player == 'O' and states[r][c] == 0 and not stop_game:
        b[r][c].configure(text='O')
        states[r][c] = 'O'
        player = 'X'
    check_for_winner()

def check_for_winner():
    global stop_game
    # Horizontal check
    for i in range(3):
        if states[i][0] == states[i][1] == states[i][2] != 0:
            b[i][0].configure(bg='grey')
            b[i][1].configure(bg='grey')
            b[i][2].configure(bg='grey')
            stop_game = True
#vertical check
    for i in range(3):
        if states[0][i] == states[1][i] == states[2][i] != 0:
            b[0][i].configure(bg='grey')
            b[1][i].configure(bg='grey')
            b[2][i].configure(bg='grey')
            stop_game = True
# Diagonal check (Top-left to Bottom-right)
  
    if states[0][0] == states[1][1] == states[2][2] != 0:
        b[0][0].configure(bg='grey')
        b[1][1].configure(bg='grey')
        b[2][2].configure(bg='grey')
        stop_game = True
# Diagonal check (Bottom-left to Top-right)

    if states[2][0] == states[1][1] == states[0][2] != 0:
        b[2][0].configure(bg='grey')
        b[1][1].configure(bg='grey')
        b[0][2].configure(bg='grey')
        stop_game = True

root = Tk()
b = [[0, 0, 0], [0, 0, 0], [0, 0, 0]]
states = [[0, 0, 0], [0, 0, 0], [0, 0, 0]]

for i in range(3):
    for j in range(3):
        b[i][j] = Button(font=('Times New Roman', 58), width=3, bg='white', 
                         command=lambda r=i, c=j: callback(r, c))
        b[i][j].grid(row=i, column=j)

player = 'X'
stop_game = False
mainloop()