SavageLab/cpCas9
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
Folders and files
Name | Name | Last commit message | Last commit date | |
---|---|---|---|---|
Repository files navigation
Order of scripts 1. trim_reads_forBWA.py : trims/QCs NGS reads for use with BWA 2. bwa_samtools.py : aligns trimmed NGS reads from (1) to premade dCas9 index and yields alignment mapping files 3. process_SAM.py : processes alignment output from (2) and produces csv w/ nucleotide mapping of CP variants 4. prep_data_for_DESeq.ipynb : converts output from (3) into a csv file w/ amino acid mapping of CP variants 5. calculate_enrichment_DESeq.R : calculates enrichment scores for CP variants using mapping from (4)
About
methods for post-sequencing analysis of circularly-permuted Cas9 libraries
Resources
Stars
Watchers
Forks
Releases
No releases published
Packages 0
No packages published