In molecular-stream separation (MSS), a stream of a multi-component mixture is separated into multiple streams of individual components inside a thin rectangular chamber. Despite great potential and many years of work on MSS, its analysis was underdeveloped until recently. To fill in this gap, we introduced a novel and convenient way to assess MSS by convoluting the separation zone into a simple 2D plot called angulagram. We implemented and publicly shared Python programs for the generation of angulagrams. However, we realized that Python programs create two hurdles (setting up a resembling Python environment and using a command line interface) for other researchers to try out and adopt this new approach. To make our approach more accessible to the MSS community, we introduce and present here the open-source software Topino for quantitative assessment of MSS. Topino is a user-friendly, accessible program with a graphical user interface that allows the user to assess MSS data in a fast and straightforward way (less than 2\ min).
Topino is available for Windows (with and without installer) as well as Debian package. Please see releases for the latest (stable) version!
- Import your MSS image by File -> Import image....
- Define your inlet using the Inlet tool
and adjust the size. - Preprocess your image
by adjusting the colour levels to obtain maximum stream-background contrast. - Proceed to the Show and edit the angulagram
view. - Evaluate the angulagram
until you get the right number of peaks (1 peak per stream) by adjusting smoothing and threshold parameters. - Export the raw data
and the angulagram 
as required.
Whether it’s your first time analyzing Molecular Stream Separations (MMS) or something that you do regularly, please take time to read the small user guide to familiarise yourself with Topino, a handy software specifically designed for MSS. Topino is a simple and intuitive software that will help you to analyze MSS pictures, extract raw data, and generate manuscript-grade figures.
Contributions are welcome!
While we are presenting here a working practical tool, we do not claim that our implementation of this tool is the best nor the only one. In fact, we foresee continuing development when other groups start adapting our approach. Hereby, we explicitly invite the community to criticize, discuss, and further develop our angulagram approach, Topino, and its implementation.