The BarBIQ pipeline is for processing the sequencing data obtained by BarBIQ method to identify Bar sequences (16S rRNA sequences) and cOTUs (cell-based taxa) and to quantify the identified cell numbers of each cOTU. This is version 1.0.0.
BarBIQ_code_1_0_0: codes; Demo: example data; Expected_output_files: expected output.
Most part of this pipeline is written in Perl (v5.22.1), and one step is written in R (version 3.5.1).
Colon/download all the files here to your local computer.
see guideline_for_BarBIQ_Pipeline.txt
Instructions for use and demo and explanation for each step, please see guideline_for_BarBIQ_Pipeline.txt
High-throughput identification and quantification of single bacterial cells in the microbiota. Nat Commun 13, 863 (2022)