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replaced DESEQ2_DEG_LIST by DEG_LIST - obtained by integrating DESeq2…
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… and edge2
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jvanheld committed Aug 19, 2018
1 parent 0b44f89 commit d83f3ea
Showing 1 changed file with 6 additions and 2 deletions.
8 changes: 6 additions & 2 deletions scripts/snakefiles/workflows/integration_ChIP_RNA.wf
Original file line number Diff line number Diff line change
Expand Up @@ -122,14 +122,18 @@ rule all:
GFF_REGULON


## Genes reported as differentially expressed by DESeq2.rules
DESEQ2_DEG_LIST=expand(expand(DEG_DIR + "/{test}_vs_{ref}/{{deg}}/{test}_vs_{ref}_{{trimming}}_{{mapping_rna}}_{{read_counts}}_{{deg}}_gene_list.txt", zip, test=rna_test, ref=rna_reference), deg=diffexpr, trimming=trimming, mapping_rna=mapping_rna, read_counts=read_counts)


## Genes reported as differentially expressed by rnaseq_deg.rules, which combies DESeq2 and edgeR
DEG_LIST=expand(expand(DEG_DIR + "/{test}_vs_{ref}/{{trimming}}_{{mapping_rna}}_{{read_counts}}_{test}_vs_{ref}_DEG_list.txt", zip, test=rna_test, ref=rna_reference), deg=diffexpr, trimming=trimming, mapping_rna=mapping_rna, read_counts=read_counts)


rule genelists_vs_regulondb:
input:
chip_genes = expand(expand(PEAKS_DIR + "/{treat}_vs_{control}/{{peakcaller}}/{treat}_vs_{control}_{{trimming}}_{{mapping_chip}}_{{peakcaller}}_gene_list.txt", zip, treat=chip_treatment, control=chip_control), peakcaller=peakcalling, trimming=trimming, mapping_chip=mapping_chip),
rna_genes = expand(expand(DEG_DIR + "/{test}_vs_{ref}/{{deg}}/{test}_vs_{ref}_{{trimming}}_{{mapping_rna}}_{{read_counts}}_{{deg}}_gene_list.txt", zip, test=rna_test, ref=rna_reference), deg=diffexpr, trimming=trimming, mapping_rna=mapping_rna, read_counts=read_counts),
rna_genes = DEG_LIST,
# rna_genes = DESEQ2_DEG_LIST,
regulondb_tus = REGULON_DIR + "/TUSet.txt",
regulondb_sites = REGULON_DIR + "/BindingSiteSet.txt",
regulondb_gene_product = REGULON_DIR + "/GeneProductSet.txt"
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