This is a small video system to monitor the development of Drosophila embryos.
| Qty | item | obs | link |
|---|---|---|---|
| 1 | 200mm makerbeam XL aluminium profiles | pieces to hold the grid that will contain the embryos aluminium profiles | |
| 2 | 100mm makerbeam XL aluminium profiles | camera mounting frame | |
| 2 | 150mm makerbeam XL aluminium profiles | camera mounting frame | |
| 4 | "L" bracket makerbeam XL | holds the aluminium profiles together | |
| 1 | 3D printed embryo grid | initially printed in black PLA | |
| 18 | M3 5mm screws | holds maker beams and other bits together | |
| 14 | M3 nuts | used together with screws |
- For this system we used Bonsai-RX to control the camera, as it allows for flexibility in determining time lapse rates and movement tracking of the embryos.
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- note: the printer used to make the grid in the images below was poorly calibrated, the final grid looks much better.
| empty | water | |
|---|---|---|
| Visible light |  |
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| Infra red light |  |
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Create a jupyter notebook to process the data
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Subtract running average from brightness average.
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square square root (so that all values are positive)
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find hatching point of the embryos (they poke out their heads at the top of the egg shell). This leads normally to a peak on the values of the traces. (maybe shape of the egg.)
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parameters that are calculated from traces:
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total movement (sum all of the values above a threshold)
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percentage time moving (number of frames where movement is above the threshold)
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average movement amplitude (average of movement above a certain threshold)
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time between first movement and hatching
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phases movement
- high frequency (slow increase and slow decrease)
- bursts of movement
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fft analysis - bin traces into 30min periods and show how frequency content changes in each bin
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test different cameras and lighting conditions
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Optogenetics led:
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LED ring turning on the red channel for 500 ms every 30 seconds.