Nucleosome sequencing analysis for Loxodes magnus

Loxodes magnus MIC and MAC nuclei were sorted with FACS. Nucleosomal DNA was prepared with Atlantis dsDNAse digest.

Workflow outline

• Map nucleosomal DNA library to Loxodes magnus MAC reference assembly (Falcon assembler)
• Also filter assembly to exclude low-complexity repeat regions previously annotated with TRF (min 1 kbp region)
• Evaluate coverage and nucleosomal DNA fragment length distribution
• Global phaseogram of nucleosome positioning relative to each other
• Phaseogram of nucleosome positioning relative to starts of gene features (TSS data not available)