The Fourier Shell Occupancy (FSO) algorithm measures the Global resolution anisotropy for cryo-EM maps, determining the quality of the map as well as infere if the particle distribution used to reconstruct the map is compatible with the resolution distributionvia directional FSC.
For a detailed information about the algorithm see our article.
#Important (May 29 2023):
The stand alone version does not provides visualization of the polar plot. It will be available in the comming days The polar plot only can be visualized in Scipion version.
There are two independent ways of using this algorithm:
- Inside Scipion (recommended): This version is much faster than the standalone version. The code is updated and provides mucho more visualization tools
- As Stand-alone application: It is slow but it does not require anything else
For shake of simplicity we encourage to use the FSO inside Scipion.
Before using the algorithm, Scipion needs to be installed. To do that, see Scipion installation instructions in the Scipion Official web site.
Once Scipipion have been installed, the Xmipp package is required, but it can be easily installed in the plugin manager inside Scipion.
Then, the algorithm will be available to be used in Scipion. You can find the algorithm searching by Ctrl+F, xmipp - resolution fso
To use the stand alone version, you only need to clone this repository and execute the installer
- Cloning the repository
git clone https://github.com/Vilax/FSO
- Installing the software. This step creates a virtual environment and install: numpy, mrcfile, matplotlib and pyqt
source installFSO.sh
- Execute FSO or
python3 fso.py
Any comment please contact us, visit the article to find our email
J.L. Vilas, H.D. Tagare, New measures of anisotropy of cryo-EM maps, Nature Methods (2023)