Compensation for fluorescence data

[phidahl]

For multichannel fluorescence measurements we need a way to compensate the fluorescence max. values.

Compensations means to take account for channel crosstalk by subtraction of an adjustable fraction of other channels from the values of one channel.

The corrections factors can be expressed by a (NxN) matrix A

fl1_max' = fl1_max - A_12 * fl2_max - A_13 * fl3_max
fl2_max' = fl2_max - A_21 * fl1_max - A_23 * fl3_max
fl3_max' = fl3_max - A_31 * fl1_max - A_32 * fl2_max

It would need a some edit fields to adjust the values of A_xy and a checkbox to switch compensation on/off.

[paulmueller]

Instead of a checkbox, would it be ok if we created new data columns for this purpose ("FL-1 maximum, cross-talk-corrected [a.u.]")?
Do you need this for the raw trace data as well?

[phidahl]

new data columns would be even better. Nope, the trace data shall be unaffected. (for now)

…

Am 26.10.2017 um 12:41 schrieb Paul Müller ***@***.***>: Instead of a checkbox, would it be ok if we created new data columns for this purpose ("FL-1 maximum, cross-talk-corrected [a.u.]")? Do you need this for the raw trace data as well? — You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub <#185 (comment)>, or mute the thread <https://github.com/notifications/unsubscribe-auth/AMPYzPWfJoOTmHjnht7LNa11lQ185-2-ks5swGHegaJpZM4QHUkc>.

[paulmueller]

This is implemented in ShapeOut 0.8.1.dev21.
Please test:
https://ci.appveyor.com/project/paulmueller/ShapeOut/build/job/hb3l29qq79d8pu64/artifacts

[phidahl]

I tested it and it seems to work.
Thank you