Elmasri_GRIN2B

Data and code for:

Elmasri M., Lotti J.S., Aziz W., Steele O.G., Karachaliou E., Sakimura, K., Hansen, K.B. and Penn, A.C. (2022) Synaptic Dysfunction by Mutations in GRIN2B: Influence of Triheteromeric NMDA Receptors on Gain-of-Function and Loss-of-Function Mutant Classification. Brain Sci. 12:789 https://doi.org/10.3390/brainsci12060789

Table of contents

Custom MATLAB functions used to analyse recording traces

./mdocs

• combiRec.m: Analyses AMPA-EPSCs and NMDA-EPSC responses from an I-V experiment
• NMDARec.m: Analyses NMDA-EPSCs measured +20 mV (in prescence of NBQX)
• ephysIO.m: Loads electrophysiology files
• wcp.m: Analysis of test pulse to calculate whole-cell recording properties
• rscomp.m: Off-line series resistance compensation
• chebexp.m: Chebyshev algorithm for fitting exponential decays

Response data used for statistical analysis

Units for peak, decay, charge, rise, dt50, fwhm, GluN1 and Homer1c are pA, ms, pC, ms, ms, ms, au and au respectively

./data

• n2b_dko_mutant_nmdar.dat
• n2b_mutant.dat
• n2b_mutant_tcn201.dat
• n2b_ko_nmdar.dat
• n2ab_C436R.dat
• n2b_hek.dat

R markdown (and knitted HTML) documents containing code and output relating to statistical analysis of the above data

./rdocs

• n2b_dko_mutant_nmdar_peak.Rmd
• n2b_dko_mutant_nmdar_charge.Rmd
• n2b_dko_mutant_nmdar_decay.Rmd
• n2b_hek_decay.Rmd
• n2b_mutant.Rmd
• n2b_mutant_tcn201_nmdar.Rmd
• n2ab_C436R.Rmd
• n2b_ko_nmdar.Rmd

Vector graphics files of graphs created in R for manuscript figures

./img