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error2 #63
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Hi,
https://bioinf.shenwei.me/seqkit/usage/#split2 best, |
thanks, that could have worked, but now I have small reads (less than 60 apparently) so it gives that error now and maybe this won't work. Do you know where can I assemble this illumina reads + pacbio clr reads? thanks again |
Hi, Best |
Hello, I want to try wengan to my yeast genome, I have Illumina adn PacBio CLR reads. My illumina reads are both 1 and 2 in one file, so I run this command
perl wengan.pl -x pacraw -a D -s illumina_reads/52530.2.367468.TTGTGTGC-TTGTGTGC.filter-RESEQ.fastq.gz -l pacbio_reads/reads.bc1015_BAK8B_OA--bc1015_BAK8B_OA.fastq.gz -p phaffia_wengan -t 20 -g 21
but some error appears
wengan-v0.2-bin-Linux/bin/abyss-pe name=phaffia_wengan.abyss2 k=96 B=20G H=4 kc=3 v=-v np=20 lib="pe1" pe1=" " contigs 2> phaffia_wengan.abyss2.err > phaffia_wengan.abyss2.log
make: *** [phaffia_wengan.mk:4: phaffia_wengan.abyss2-contigs.fa] Error 2
any suggestion? thanks
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