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I'm trying to generate a custom genome of all protein coding genes in hg38. The fasta file I generated is structured in a way were each gene is an individual record instead of each chromosome. Overall, it's like I have 25k different "chromosomes". I then wanted to create a custom genome from this fasta file to use for mapping. To do this I've used the command below:
I calculated both the --genomeSAindexNbases and --genomeChrBinNbits based on the STAR manual. However, whenever I try to run genomeGenerate, STAR always hangs up at 'sorting Suffix Array chunks and saving them to disk' and just sits there for days. Is there something that I'm doing wrong? Any suggestions on how I may be able to get this to work? I'm desperate for any tips. I've been trying for days to get this to work, including trying to increase RAM to 128GB and Threads to 12 by running in on aws batch. I've attached my log.out file for reference
since the "genome" sequence may be repetitive, this option might help to speed up genome generation: --genomeSuffixLengthMax 300. The length here is the maximum read length you are planning to map.
I'm trying to generate a custom genome of all protein coding genes in hg38. The fasta file I generated is structured in a way were each gene is an individual record instead of each chromosome. Overall, it's like I have 25k different "chromosomes". I then wanted to create a custom genome from this fasta file to use for mapping. To do this I've used the command below:
I calculated both the --genomeSAindexNbases and --genomeChrBinNbits based on the STAR manual. However, whenever I try to run genomeGenerate, STAR always hangs up at 'sorting Suffix Array chunks and saving them to disk' and just sits there for days. Is there something that I'm doing wrong? Any suggestions on how I may be able to get this to work? I'm desperate for any tips. I've been trying for days to get this to work, including trying to increase RAM to 128GB and Threads to 12 by running in on aws batch. I've attached my log.out file for reference
Log.out.txt
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