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Segfault with a particular read agains small genome #20

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tdido opened this issue Feb 6, 2015 · 2 comments
Closed

Segfault with a particular read agains small genome #20

tdido opened this issue Feb 6, 2015 · 2 comments

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@tdido
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tdido commented Feb 6, 2015

Up to 2.4.0j I was suffering from issue #17. Now I'm able to build my genome, but I've identified a single particular read that causes a segfault when aligning. Test data here: https://gist.github.com/tdido/55c94c003c21b4f3620d. Run parameters are as follows:

STAR --runMode genomeGenerate --genomeDir genome/ --genomeFastaFiles genome.fasta
STAR --genomeDir genome/ --readFilesIn test_single_fail.fastq

Additionally, if you change one of the first 'A's in the poly-A stretch of the read to any other nucleotide, the alignment completes with no problems.

@alexdobin
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Hi,
this is an old issue with small genomes in STAR, please see the following post:
https://groups.google.com/forum/#!msg/rna-star/vNB4_I1FU2k/RcWqPRrXxj4J
If you use --genomeSAindexNbases 5 at the genome generation step, the error at the mapping stage should disappear.

Cheers
Alex

@tdido
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tdido commented Feb 20, 2015

Ah, I see. Thanks for the reference. Is the mailing list the best place to try and track open bugs, or do you use some other tracker?

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