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SMA - Single Molecule Analysis BDA 10/22/15 UNDER DEVELOPMENT Updated 2/22/16 - developing for 2 channel data. Done, lightly tested on bead data. map_coords works; warping the image is either right or very close. Dependencies - numpy, scipy, Matplotlib, PIL https://github.com/ZhuangLab/storm-analysis Also requires yattag - www.yattag.org OpenCV -- http://opencv.org/ This code is run in several steps: -[TO DO: For multi channel data: generate bead mapping] -Find peaks, either in the beginning of the movie (typically for smFRET data) or throughout the entire movie (typically for ORBIT data). Output .pks or .pks3d file. Currently only .pks3d. -Analyze those peaks: intensities, and optionally fits. Output either as .traces file (for smFRET data) or as .trdir directory of single trace files (for ORBIT data). Currently only .trdir. Major pieces: -ffpdax: find peaks in .dax movie. Can be set to automatically run apdax when done. -apdax: analyze those peaks. -single xml file specifies settings for both codes. -multibatch - run multiple analyses in parallel. (Or, for better output, run each in a separate command line window) Functional and tested for a very limited set of settings (for now). Works with: .pks3d, emchs=1 or 2, .trdir, ALEX4 = 0 or 1
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